乙型肝炎病毒YMDD变异株的检测及其临床意义  

Detection of YMDD mutations in hepatitis B virus and analysis of its clinical significance

作　　者：方莉[1] 雷娇[2] 赵维皎[1] 陈莹[1] 

机构地区：[1]川北医学院附属医院检验科,四川南充637000 [2]川北医学院医学检验系,2009级四川南充637000

出　　处：《国际检验医学杂志》2015年第15期2197-2198,2201,共3页International Journal of Laboratory Medicine

摘　　要：目的：探讨慢性乙型肝炎患者 HBV YMDD变异的情况、相关影响因素及临床意义，为临床诊断和治疗提供依据。方法对100例慢性乙型肝炎患者采用实时荧光PCR法检测血清中HBVYMDD变异及HBVDNA载量。结果100例乙肝患者中检出HBV YMDD变异共13例（阳性率13％），其中YIDD变异7例，YVDD变异5例，YIDD／YVDD共生变异1例。13例YMDD变异患者 HBV DNA在（1．77×105～1×108） IU／mL ，丙氨酸转氨酶（ALT ）水平18．1～273．5 U／L。结论未发现慢性乙型肝炎患者的性别、年龄、乙型肝炎E抗原（HBeAg）状态、HBV DNA载量及ALT水平与 HBV YMDD变异检出率之间存在相关性，差异无统计学意义（P＞0．05）。Objective To discuss YMDD mutation ,related factors and clinical significance in chronic hepatitis B patients ,provi‐ding the basis for clinical diagnosis and treatment .Methods YMDD mutations and DNA load of hepatitis B virus in 100 chronic hepatitis B patients were detected by real‐time fluorescence PCR .Results YMDD mutations of hepatitis B virus were found in 13 out of 100 patients（positive rate 13% ） .Including YIDD mutations in 7 ,YVDD mutations in 5 and YIDD/YVDD symbiotic mutation in 1 .In the 13 patients ,HBV DNA load and ALT level were at （1 .77 × 105 -1×108 ）IU/mL and 18 .1-273 .5 U/L .Conclusion Do not find the detection rate of YMDD mutation in chronic hepatitis B patients is related with gender ,age ,HBeAg status ,HBV DNA load and ALT level ,the difference has no statistical significance （P〉0 .05） .

关 键 词：聚合酶链反应 慢性乙型肝炎 变异 

分 类 号：R512.62[医药卫生—内科学]