苦瓜总皂苷对改善2型糖尿病大鼠胰岛素抵抗关键因子的影响  

Effect of the momordica charantia total saponin on the key factors of ameliorating insulin resistance in type 2 diabetes mellitus rats

作　　者：马春宇[1] 于洪宇[2] 王慧娇[1] 耿丽晶[3] 

机构地区：[1]辽宁医学院实验诊断学教研室,辽宁锦州121000 [2]辽宁医学院护理学院,辽宁锦州121000 [3]辽宁医学院食品学院,辽宁锦州121000

出　　处：《中国临床药理学杂志》2015年第15期1522-1525,共4页The Chinese Journal of Clinical Pharmacology

基　　金：辽宁省自然科学基金项目资助(2014022043)

摘　　要：目的通过苦瓜总皂苷对2型糖尿病大鼠蛋白激酶B(PKB或Akt-2)和腺苷酸活化蛋白激酶(AMPK)的作用来揭示其改善胰岛素抵抗的机制。方法用高脂高糖饮食喂养结合小剂量链脲佐菌素注射,来复制2型糖尿病模型。按照体重随机分为6组:正常组、模型组、对照组和3个剂量实验组,对照组和3个剂量实验组大鼠每天分别给予50 mg·kg-1二甲双胍2 m L和3个剂量(100,200,400 mg·kg-1)苦瓜总皂苷2 m L,灌胃8周。用生化分析仪检测空腹血糖,用酶联免疫吸附试验检测胰岛素,用RT-PCR和Western Blot法检测肝组织Akt-2和AMPK的mRNA和蛋白表达量。结果 3个剂量实验组稳态胰岛素抵抗评价指数较模型组明显降低,Akt-2和AMPK的mRNA和蛋白表达量较模型组明显增高。结论苦瓜总皂苷改善胰岛素抵抗的作用机制可能为上调Akt-2和AMPK的mRNA以及蛋白表达量。Objective To study the effect mechanism of momordica charantia total saponin （MCTS）＇s ameliorating insulin resistance through discussing MCTS＇ s influence of protein kinase B （Akt -2 ） and adenosine monophosphate activated protein kinase （AMPK） in type 2 diabetes（T2DM） rats. Methods T2DM models were built with the method of higher lipid and sugar diets and injection of a low dose of streptozotocin. Rats are divided into normal group, model group, control group and three dosage test groups. The positive control group and three dosage test groups rats were administrated with 2 mL metformin （50 mg· kg^-1） and MCTS （100, 200, 400 mg · kg^-1） per day for8 weeks. Fasting serum glucose and insulin were detected with biochemical analyzer and ELISA method. The Akt- 2 and AMPK＇s mRNA and the activated protein were measured by RT- PCR and Western blot method. Results The homeostasis model assessment of insulin resistance （HOMA -IR） reduced and the mRNA and protein expression amount of Akt -2 and AMPK increased obviously in all test groups compared to the model group. Conclusion The ameliorating insulin resistance mecha- nism of MCTS maybe caused by up - regulating the mRNA and protein of Akt- 2 and AMPK.

关 键 词：苦瓜总皂苷 2型糖尿病 胰岛素抵抗 蛋白激酶B 腺苷酸活化蛋白激酶 

分 类 号：R285.5[医药卫生—中药学] R977.15[医药卫生—中医学]