格列喹酮干预治疗对糖尿病大鼠肾脏组织及核因子-κB与炎症因子表达影响的观察  

Effects of Gliquidone treatment on kidney tissue and the expression of nuclear factor-κB and inflammatory cytokines in diabetic rats

作　　者：闫双通[1] 李春霖[1] 陆菊明[2] 田慧[1] 肖彧君[1] 刘瑜[1] 谷昭艳[1] 

机构地区：[1]解放军总医院老年内分泌科,北京100853 [2]解放军总医院内分泌科,北京100853

出　　处：《中国糖尿病杂志》2015年第8期742-746,共5页Chinese Journal of Diabetes

基　　金：国家自然科学基金(81173625;30873412;30801201)

摘　　要：目的探讨格列喹酮干预治疗对糖尿病肾脏病变中肾小球病理改变的影响以及对核因子-κB(NF-κB)、环氧合酶-2(COX-2)和诱导型一氧化氮合酶(iNOS)表达的影响。方法将8月龄雄性Wistar大鼠24只分为正常对照(NC)组、糖尿病(DM)组和格列喹酮干预(GI)组,每组8只。NC组予普通饮食喂养,糖尿病大鼠采用高糖高脂饮食及腹腔注射STZ制备。GI组在成模后予格列喹酮喂养干预。喂养2月后处死,留取各组肾脏标本,观察肾脏病理改变。采用分子生物学方法检测肾脏组织NF-κB、COX-2和iNOS表达情况。结果与NC组相比,DM、GI组肾脏均有不同程度受损表现,其中,DM组病理改变最明显。与NC组相比,DM、GI组肾脏组织NF-κB、COX-2和iNOS表达均增加,GI组表达低于DM组(P<0.05)。结论 NF-κB激活及其启动的下游炎症因子在DN发生发展过程中可能起重要作用,格列喹酮干预治疗可抑制NF-κB激活及相应炎症因子表达,间接起到肾脏保护作用。Objective To investigate the effect of gliquidone interference treatment on the glomerular pathological change in the diabetic nephropathy ,as well as on the expressions of nuclear factor‐κB（NF‐κB） ,COX‐2 ,iNOS. Methods 24 eight‐months male wistar rats were divided into normol control （NC） group ,diabetic （DM ） group and gliquidone interference （GI） group. The rats in NC group were fed with common diet. Diabetes was induced by feeding with high fat and sucrose diet plus intraperitoneal injection of STZ . The feed of diabetic rat was added with gliquidone in the gliquidone interference group. The rats were executed after two‐months feeding ,and the specimens of kidney of the rats in each group were obtained so as to observe the pathological changes. The expressions of NF‐κB ,iNOS and COX‐2 were analyzed by molecular biology method. Results Compared with NC group ,there were varying degrees of kidney lesions in DM group and GI group.Compared with NC group ,the expressions of NF‐κB , COX‐2 and iNOS in the kidney increased significantly in DM group and GI group. Compared with DM group ,the expression was milder in GI group （P〈0.05）. Conclusion NF‐κB and downstream target gene were activated in the diabetic rats. Treatment with gliquidone can attenuate the expression of NF‐κB and dow nstream target gene.

关 键 词：糖尿病 格列喹酮 肾脏 炎症因子 大鼠 

分 类 号：R587.1[医药卫生—内分泌]