山西晋城马铃薯疮痂病病原菌鉴定  

Identification of The Pathogen of Potato Scab in Jincheng of Shanxi Province

作　　者：王丹[1] 姚晓东[1] 李新凤[1] 王美琴[1] 郝晓娟[1] 王建明[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2015年第5期495-498,共4页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金青年基金(31000873);山西农业大学中青年学术骨干基金(XG201210);山西省科技攻关项目(20120311016-2)

摘　　要：为分离鉴定山西晋城马铃薯疮痂病病原菌,对分离物进行致病性测定、形态学观察、生理生化特性测定及16S rDNA序列分析。结果表明,该分离物在燕麦培养基上菌落呈灰白色,边缘稍有褶皱突起,显微观察孢子呈灰色,孢子链呈螺旋状,结合生理生化特性测定结果将其鉴定为Streptomyces scabies。该菌株与Streptomyces scabies(登录号为HM018114)的16SrDNA序列同源性为100%。引起山西晋城马铃薯疮痂病的病原菌为Streptomyces scabies。The pathogen causing potato scab in Jincheng of Shanxi province was isolated and identified. The isolated strain from potato scab was analyzed and identified by pathogenicity identification ,morphological observation ,determi‐nation of physiological and biochemical characteristics and the analysis of 16S rDNA sequence. The colony of isolate was grey ,which showing wrinkle and bulge on the edge of the colony.Conidium were grey and spore chains were spi‐ral. According to morphological features and the charactersitics of physiology and biochemistry ,the isolate was identi‐fied as Streptomyces scabies. The results of 16S rDNA sequences of the isolated strain showed 100% identity withStreptomyces scabies （HM018114）. The results showed that the pathogen causing scab in Jincheng of Shanxi prov‐ince wasStreptomyces scabies.

关 键 词：马铃薯疮痂病 病原菌鉴定 链霉菌 16S RDNA 序列 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]