635nm/808nm双波长低能量激光对人成纤维细胞氧化应激反应的研究  

Research of Oxidative Stress Reaction Induced by 635 nm/808 nm Double Wavelength Low Level Laser Therapy on CCC-ESF

作　　者：陈洪丽[1] 王宏[1] 王超[2] 阴慧娟[1] 金文东[1] 穆志明[1] 李迎新[1] 

机构地区：[1]中国医学科学院北京协和医学院生物医学工程研究所,天津300192 [2]天津医科大学生物医学工程学院,天津300070

出　　处：《激光与光电子学进展》2015年第8期247-253,共7页Laser & Optoelectronics Progress

基　　金：协和青年基金(33320140137;33320140056)

摘　　要：研究和比较双波长低能量激光疗法(LLLT)对人皮肤成纤维细胞(CCC-ESF)的生长、酶活性以及生长因子等的影响,讨论弱激光促进伤口愈合的作用机理。体外培养CCC-ESF,实验分为对照组和照光组(635 nm组、808 nm组和635 nm/808 nm组),照光剂量为20 m W/cm2、12 J/cm2,分别检测细胞增殖情况、活性氧(ROS)产量、抗氧化酶活性[过氧化氢酶(CAT)和超氧化物歧化酶(SOD)]、脂质氧化能力(MDA)以及细胞生长因子含量。635 nm组细胞增殖速度明显大于其他各组;635 nm、808 nm和635 nm/808 nm组ROS水平分别比对照组提高了14.55%、7.49%和14.92%;CAT和SOD酶活力提高;808 nm激光促进MDA增加;双波长激光使人白介素1(IL-1)和成纤维细胞生长因子(FGF)分泌量显著增多;808 nm激光增加了人胰岛素样生长因子(IGF-1)和人胶质细胞源性神经营养因子(GDNF)的分泌。LLLT诱导产生的低水平氧化应激反应可促进CCC-ESF增殖,提升细胞活力,增强细胞对外界环境的抵抗能力。LLLT刺激细胞分泌多种细胞因子,调控创伤愈合的进程。双波长激光产生的生物效应优于单一波长。To investigate and compare the biological effect of proliferation, enzyme activity and growth factor after double wavelength low level laser therapy （LLLT） on human embryonic skin fibroblasts （CCC-ESF） in vito, and explore the molecular mechanism of wound healing, in vitro culture of CCC-ESF is divided into control group and experimental groups （635 nm group, 808 nm group, 635 nm/808 nm group）, laser of 20 mW/cm2, 12 J/cm2 is applied to detect cell proliferation, reactive oxygen species （ROS） production, antioxidant enzyme activity [catalase （CAT） and superoxide dismutase （SOD）], lipid oxidation （MDA） and amount of cytokines related. Cell proliferation rate in the 635 nm group is significantly higher than that in other groups; ROS levels are 14.55%, 7.49% and 14.92% respectively in 635 nm, 808 nm and 635 nm/808 nm groups, which are higher than that in the control group; CAT and SOD enzyme activity increases after LLLT; 808 nm laser promotes the level of MDA and expression of interleukine- 1 （IL- 1） and fibroblast growth factor （FGF）; 808 nm laser can stimulate the secretion of insulin-like growth factor 1 （IGF-1） and glial cell line-derived neurotrophic factor （GDNF）. Low levels of oxidative stress induced by LLLT can promote the proliferation of CCC-ESF, improve cell viability, and enhance cellular resistance to the external environment. LLLT stimulates the secretion of a variety of cytokines and regulates the wound healing process. Biological effect produced by double wavelength LLLT is better than that with a single wavelength in CCC-ESF.

关 键 词：医用光学 低能量激光 成纤维细胞 活性氧 生长因子 

分 类 号：R318.51[医药卫生—生物医学工程]