白藜芦醇对慢性癫痫大鼠脑组织中氨基酸类递质含量的影响  

Effects of resveratrol on the changes of amino acids level in brain tissues in chronic epilepsy rats

作　　者：李敏[1] 潘群皖[1] 王海华[1] 孔德虎[2] 王烈成[2] 

机构地区：[1]皖南医学院生理学教研室,安徽芜湖241002 [2]安徽医科大学生理学教研室,安徽合肥230032

出　　处：《皖南医学院学报》2015年第4期307-309,313,共4页Journal of Wannan Medical College

基　　金：国家自然科学基金面上项目(81071075);安徽省教育厅自然科学重点基金项目(KJ2007A028)

摘　　要：目的:测定慢性癫痫大鼠脑组织谷氨酸(Glu)和γ-氨基丁酸(GABA)含量,分析白藜芦醇抗癫痫作用机制。方法:动物分为生理盐水组(NS组)、癫痫组(KA组)、低剂量白藜芦醇组(L-Res组)和高剂量白藜芦醇组(H-Res组)。利用立体定位技术,在大鼠右侧海马CA3中心区注射海人草酸,建立癫痫大鼠模型,以高、低剂量白藜芦醇干预10 d,利用高效液相色谱法测定慢性癫痫大鼠海马和皮层Glu和GABA含量。结果:与NS组比较,KA组大鼠海马和皮层Glu含量、Glu/GABA比值均明显升高(P<0.05);与KA组比较,L-Res组大鼠海马Glu含量、Glu/GABA比值均明显降低(P<0.05),但皮层Glu含量、Glu/GABA比值却明显高于NS组(P<0.05);H-Res组大鼠海马和皮层Glu含量、Glu/GABA比值均明显高于NS组(P<0.05)。结论:白藜芦醇通过维持海马Glu/GABA平衡达到抗癫痫作用效果。Objective：To investigate the mechanisms of resveratrol in anti-epilepsy through determining the contents of glutamic acid （ Glu） and γ-ami-nobutyric acid （GABA） in rats with chronic epilepsy.Methods：Animals were divided into saline group （NS group）, epilepsy group （KA group）, low-dose resveratrol group （ L-Res group） and high-dose resveratrol group （ H-Res group） .Animal models of epilepsy were induced by injection of kainic acid （KA） into the center of the CA3 region of the right hippocampus with stereotaxic technology.Resveratrol was intragastrically administered immediately fol-lowing the first onset of seizure, once a day, for consecutive 10 days.Hight performance liquid chromatography（ HPLC） was performed to detect the con-tents of Glu and GABA in hippocampus and cortex of model rats.Results：Compared with NS group, the contents of Glu and the ratio of Glu/GABA in hippocampus and cortex were increased significantly in KA group （P〈0.05）, whereas the contents of Glu and the ratio of Glu/GABA in hippocampus was decreased in rats treated with L-Res group compared to KA group（P〈0.05）, and the rats treated with H-Res group had higher level of Glu and ratio of Glu/GABA in cortex and in hippocampus（P〈0.05）.Conclusion：Resveratrol may produce anti-epileptic effects by reversing the imbalance between Glu and GABA in hippocampus in chronic epilepsy rats.

关 键 词：高效液相色谱 氨基酸 癫痫 白藜芦醇 

分 类 号：R285.5[医药卫生—中药学]