基于样本熵的乌拉坦麻醉大鼠脑电特征初步研究  

Pilot study on EEG feature extraction of urethane-anesthetized rats based on sample entropy

作　　者：高春芳[1] 张根葆[2] 李田[1] 陆晓华[3] 黄磊[1] 徐晓燕[1] 张娅[2] 

机构地区：[1]皖南医学院医学工程学教研室,安徽芜湖241002 [2]皖南医学院病理生理学教研室,安徽芜湖241002 [3]皖南医学院机能实验中心,安徽芜湖241002

出　　处：《皖南医学院学报》2015年第4期314-316,321,共4页Journal of Wannan Medical College

基　　金：皖南医学院中青年科研基金项目(WK201419)

摘　　要：目的:初步探究样本熵值在大鼠不同麻醉状态中的差异,期望能用样本熵值描述麻醉深度。方法:SD大鼠随机分为3组:常规麻醉剂量组(S组),减半剂量伴小剂量追加给药组(HL组),常规剂量伴小剂量追加给药组(SL组),每组7只。用RM6240生物信号采集处理系统采集麻醉大鼠头皮脑电。应用MATLAB软件编写样本熵程序对不同组麻醉大鼠头皮脑电信号做非线性动力学样本熵分析。结果:S组脑电样本熵均值从2.8降低到2.4,有明显的降低阶段,但这个低熵值不能长期维持。HL组样本熵均值从3.8降低到2.6,起始熵值较高且下降速度缓慢。SL组脑电样本熵均值从2.8降低到1.9,在小剂量追加给药期间能长期维持低样本熵状态。在3组内每只大鼠低样本熵时段取2段脑电数据(每组共14段),做组间样本熵值的单因素方差分析,P<0.01,有显著性差异。结论:3种给药情况下,大鼠在行为学上都能够进入昏睡、尾部刺痛无明显应激反应的麻醉状态,但脑电的样本熵值有显著性差异。提示样本熵值比行为学观察能更精细地监测大鼠麻醉深度。Objective：To preliminarily investigate the variation of sample entropy （ SampEn） in rats in different anesthesia state for accurate definition of the anesthesia depth.Methods：Sprague-Dawley（ SD） rats were randomized into 3 groups,namely：standard anesthetic dosing（ group S） ,the first half dos-age with incremental small dose of urethane（group HL） and standard dosage with adding small dose of urethane（group SL）（n=7 for each）.RM6240 ap-paratus was used to acquire the EEGs of rats,and MATLAB software,to program the sample entropy for EEGs extracted in rats anesthetized with different dose of urethane.SampEn of EEG at individual time domain was calculated and analyzed based on non-linear dynamics.Results：Mean SampEn in group S was reduced to 2.4 from 2.8,with noticeably depressed time domain,yet it failed to sustain for long.Mean SampEn of group HL was declined to 2.6 from 3.8,and descended slowly from initiative higher value.Fall of mean SampEn was seen in group SL（1.9 from 2.8）,and the SampEn was maintained low over a long period of time during small dosing increment.One-way ANOVA test showed significant difference between individual SampEn value with regard to the two EEG segments（total 14 segments in each group） in low entropy in the three groups （P〈0.01）.Conclusion：The three dosing of urethane can drive the experimental rats into drowsy state and freedom of pain response to the needling at the tail.However,EEG SampEn varies a lot,suggesting that SampEn may be better measurement than behavioral observation in monitoring the depth of sedation .

关 键 词：样本熵 乌拉坦 脑电 麻醉深度 

分 类 号：R614[医药卫生—麻醉学]