富氢液对神经病理性痛大鼠坐骨神经及脊髓炎性反应的影响  

Effects of hydrogen-rich saline on inflammatory responses in sciatic nerve and spinal cord of rats with neuropathic pain

作　　者：陈亚军[1] 陈红光[1] 于泳浩[1] 

机构地区：[1]天津医科大学总医院麻醉科天津市麻醉学研究所,300052

出　　处：《中华麻醉学杂志》2015年第4期460-462,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81372033,81101409）;天津市应用基础及前沿技术研究计划资助项目（11JCYBJC12900）;天津市卫生局资助项目（2011KZ108）

摘　　要：目的 评价富氢液对神经病理性痛大鼠坐骨神经及脊髓炎性反应的影响.方法 健康成年雄性SD大鼠90只,体重180～220 g,采用随机数字表法分为3组（n=30）：假手术组（S组）、神经病理性痛组（NP组）和富氢液组（H组）.采用结扎坐骨神经的方法制备大鼠神经病理性痛模型.H组于结扎坐骨神经后腹腔注射富氢液（0.6 mmol/L）10 ml/kg,2次/d,共7d,S组和NP组给予等容量生理盐水.分别于结扎坐骨神经后7、10和14 d时测定机械痛阈和热痛阈,随后处死大鼠取脊髓和结扎侧坐骨神经,采用ELISA法检测TNF-α、IL-1β和高迁移率族蛋白1（HMGB1）的含量.结果 与S组比较,NP组和H组各时点机械痛阈和热痛阈降低,坐骨神经和脊髓TNF-α、IL-1β和HMGB1含量升高（P＜0.05）;与NP组比较,H组各时点机械痛阈和热痛阈升高,坐骨神经和脊髓TNF-α、IL-1β和HMGB1含量降低（P＜0.05）.结论 富氢液通过抑制坐骨神经及脊髓炎性反应,减轻大鼠神经病理性痛.Objective To evaluate the effects of hydrogen-rich saline on inflammatory responses in the sciatic nerve and spinal cord of rats with neuropathic pain （NP）.Methods Ninety male adult Sprague-Dawley rats,weighing 180-220 g,were randomly divided into three groups （n=30 each） using a random number table：sham operation group （group S）;NP group;hydrogen-rich saline group （group H）.NP was induced by chronic constrictive injury.The animals were anesthetized with intraperitoneal 10％ chloral hydrate 300 mg/kg.The left sciatic nerve was exposed and ligated with 4-0 silk at 1 mm intervals.After ligation of the sciatic nerve,hydrogen-rich saline （0.6 mmol/L） 10 ml/kg was injected intraperitoneally twice a day for 7 consecutive days in group H,and the equal volume of normal saline was given in S and NP groups.At 7,10 and 14 days after ligation,the mechanical paw withdrawal threshold （MWT） and thermal paw withdrawal latency （TWL） were measured.The rats were then sacrificed,and the left sciatic nerve and L4-6 segments of the spinal cords were removed for detection of the contents of tumor necrosis factor-alpha （TNF-α）,interleukin-1 beta （IL-1β） and high mobility group box-1 （HMGB1） at 7,10 and 14 days after ligation.Results Compared with group S,the MWT and TWL were significantly decreased,and the contents of TNF-α,IL-1β and HMGB1 in the left sciatic nerve and spinal cords were increased at each time point in NP and H groups.Compared with group NP,the MWT and TWL were significantly increased,and the contents of TNF-α,IL-1β and HMGB1 in the left sciatic nerve and spinal cords were decreased at each time point in group H.Conclusion Hydrogen-rich saline alleviates NP through inhibiting inflammatory responses in the sciatic nerve and spinal cord of rats.

关 键 词：氢 神经痛 炎症 坐骨神经 脊髓 

分 类 号：R614[医药卫生—麻醉学]