异丙酚对大鼠脑缺血再灌注时神经元线粒体DNA缺失的影响  

Effect of propofol on mitochondrial DNA deletion in neurons during brain ischemia-reperfusion in rats

作　　者：王亚芳[1] 张睿[1] 刘莹[1] 岳子勇[1] 董弘[1] 杨万超[1] 钱华[1] 宋春雨[1] 

机构地区：[1]哈尔滨医科大学附属第二医院麻醉科黑龙江省麻醉与危重病学研究重点实验室,150086

出　　处：《中华麻醉学杂志》2015年第4期493-495,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金青年科学基金（81301668）

摘　　要：目的 评价异丙酚对大鼠脑缺血再灌注时神经元线粒体DNA缺失的影响.方法 雄性SD大鼠54只,3月龄,体重250～300 g,采用随机数字表法分为3组（n=18）：假手术组（S组）、脑缺血再灌注组（I/R组）和异丙酚组（P组）.采用夹闭双侧颈总动脉联合低血压的方法建立大鼠脑缺血再灌注损伤模型.P组股静脉输注异丙酚1.0 mg·kg-1·min-11h,然后制备脑缺血再灌注损伤模型.分别于再灌注6、24和48 h时,每组处死6只大鼠,取脑组织,光镜下观察病理学结果;分离海马组织,提取神经元线粒体DNA,采用RT-PCR法检测线粒体DNA缺失率.结果 与S组比较,I/R组再灌注6、24和48 h时线粒体DNA缺失率升高,P组再灌注48 h时线粒体DNA缺失率升高（P＜0.05）;与I/R组比较,P组再灌注6、24和48 h时线粒体DNA缺失率降低（P＜0.05）,脑组织病理学损伤减轻.结论 异丙酚减轻大鼠脑缺血再灌注损伤的机制与减少神经元线粒体DNA缺失有关.Objective To evaluate the effect of propofol on mitochondrial DNA （mtDNA） deletion in neurons during brain ischemia-reperfusion （I/R） in rats.Methods Fifty-four male Sprague-Dawley rats,aged 3 months,weighing 250-300 g,were randomly divided into 3 groups （n =18 each） using a random number table：sham operation group （S group）,I/R group and propofol group （P group）.Brain I/R was produced by occlusion of bilateral common carotid arteries combined with hypotension.In group P,propofol was infused for 1 h at 1.0 mg · kg-1 · min-1 via the femoral vein before I/R.At 6,24 and 48 h of reperfusion,6 rats were sacrificed,and brains were removed for examination of pathologic changes with light microscope.Hippocampi were isolated,and mtDNA was extracted to detect mtDNA deletion using real-time PCR.Results Compared with group S,mtDNA deletion at 6,24 and 48 h of reperfusion in group I/R,and at 48 h of reperfusion in group P were significantly increased.Compared with group I/R,mtDNA deletion was significantly decreased at 6,24 and 48 h of reperfusion,and the pathologic changes of brains were mitigated in group P.Conclusion The mechanism by which propofol reduces global brain I/R injury is related to reduction of mtDNA deletion in neurons of rats.

关 键 词：二异丙酚 再灌注损伤 脑 神经元 DNA 线粒体 

分 类 号：R614[医药卫生—麻醉学]