大鼠脊髓半横断损伤后S-100B表达的变化  

Changes in S-100B Expression of Astrocytes after Experimental Hemi-sectioned Spinal Cord Injury of Rats

作　　者：邹云[1] 余资江[1] 林金棠 

机构地区：[1]贵州医科大学,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第9期900-904,共5页Journal of Guiyang Medical College

基　　金：国家自然科学基金项目(81060108)

摘　　要：目的:研究大鼠脊髓半横断损伤(SCI)后损伤区域星形胶质源性蛋白(S-100B)的表达情况。方法:将70只雄性成年SD大鼠随机分为正常组(10只)、假手术组(10只)及模型组(行T10脊髓右侧半切术,术后分为1 d,7 d,14 d,21 d,28 d组,每组各10只);术后于相应时间点采用BBB及Reuter法行后肢神经功能评分,评分后处死大鼠,对SCI后损伤区域行S-100B免疫组织化学染色,Western blot检测S-100B蛋白表达,透射电镜观察损伤区域的组织形态学变化。结果:(1)BBB与Reuter评分显示模型组大鼠麻醉清醒后即出现瘫痪及感觉功能障碍,术后7 d神经功能开始好转,随着时间的推移,神经功能逐渐恢复,14 d时后肢神经功能恢复最明显;(2)免疫组织化学显示正常组及假手术组少量表达S-100B,模型组S-100B表达较正常组及假手术组均增高,S-100B表达在14 d时达到高峰,大量反应性星形胶质细胞增生、肥大,28 d时S-100B表达趋于稳定;(3)Western blot检测S-100B蛋白表达水平与免疫组织化学结果相一致;(4)电镜下SCI后损伤区域神经元肿胀,变性,细胞器减少,线粒体水肿,空泡变性。结论:SCI后S-100B表达增高,星形胶质细胞增生,可能与损伤神经的自发修复过程有关。Objective： To investigate the expression of S-100B protein of astrocytes after spinal cord injury （SCI）. Methods： Seventy healthy adult male SD rats were selected and randomly divided into 3 groups ： normal control group, sham group and model of SCI group （ 1 d, 7 d, 14 d, 21 d and 28 d af- ter SCI）. After the establishment of animal model of SCI, the functional recovery of the hind limb was evaluated by using BBB （Basso, Beattie and Bresnahan locomotor rating scale）and Reuter score at 1 d, 7 d, 14 d, 21 d and 28 d. After hind limb function was scored, the rats were sacrificed to under- go S-100B immunohistochemistry staining. Western blot was adopted to detect the expression of S-100B protein in damaged area and transmission electron microscope to observe the morphological change of spinal cord after SCI in rats Results： （1）In BBB and Reuter score evaluation, the rats of SCI model groups paralyzed and showed sensory disturbance after anesthetic awareness. The neurologic function of the SCI rats＇hind limb began to recover gradually, with the recovery being most obvious at 14thd. （2） HE staining showed that S-100B protein expression in each experimental groups were higher than that of normal control and sham group, with S-100B protein expression reaching the highest level at 14th d. The astrocyte in the SCI area proliferated arid hypertrophied. At 28th d S-100B protein expression ten- ded to be stable. （3）The detection of S-100B protein expression by Western blot was consistent with result of immunohistochemistry staining. （4） Under transmission electron microscope, in injured area after SCI, the neurons swelling and degeneration, the decreased number of cell organelle, mitochondrial edema and vacuolar degeneration could be observed. Conclusion： After SCI, the increased S-100B protein expression and astrocyte proliferation may be related to self-repair and regeneration of injured nerve.

关 键 词：脊髓损伤 修复 神经再生 星形胶质源性蛋白 大鼠 Sprague-Dawley 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]