大鼠脊髓半横断损伤后胶质细胞反应性增生的变化规律及意义  

The Significance of Proliferation of Glial Cells Induced by Experimental Hemi-sectioned Spinal Cord Injury

作　　者：邹云[1] 余资江[1] 林金棠 

机构地区：[1]贵州医科大学,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第9期905-909,共5页Journal of Guiyang Medical College

基　　金：国家自然科学基金项目(81060108)

摘　　要：目的：探讨脊髓损伤（SCI）后神经胶质细胞反应性增生的变化规律及其在损伤修复中的可能机制。方法：将70只雄性成年SD大鼠随机分为正常组（10只）、假手术组（10只）及模型组（行T10脊髓右侧半切术,术后分为1,7,14,21及28 d组,每组各10只）,术后于相应时间点采用BBB法行后肢神经功能评分,评分后处死大鼠,分别行HE染色及免疫组化染色,观察各组大鼠SCI后的损伤部位脊髓的组织形态学变化,GFAP及MBP阳性细胞。结果：（1）BBB评分,模型组大鼠麻醉清醒后即出现瘫痪,术后7 d伤侧后肢运动功能开始好转,神经功能逐渐恢复,14 d时恢复最明显;（2）HE染色,模型组大鼠术后1 d可见损伤区域弥漫性出血,神经元变性、坏死,7 d时炎性细胞浸润,14~21 d时囊腔形成,术后28d脊髓损伤处被瘢痕组织替代;（3）GFAP阳性细胞,模型组GFAP表达较正常组及假手术组均增加,星形胶质细胞增生、肥大,术后14 d星形胶质细胞增生达到高峰;（4）MBP阳性细胞,模型组术后1 d MBP表达明显降低,损伤区域内髓鞘破坏,术后7 d轴突间隙增宽,MBP表达开始升高,术后14 d轴突间隙扩大。结论：SCI后,大鼠后肢神经功能的恢复可能与星形胶质细胞及少突胶质细胞反应性增生有关。Objective： To explore the change rule of neurogliocyte reactive hyperplasia and its possi- ble mechanism in damage repair after spinal cord injury （SCI）. Methods： Seventy healthy adult male SD rats were selected and randomly divided into 3 groups： normal control group, sham group and SCI model groups （ 1 day after SCI, 7 day after SCI, 14 day after SCI, 21 day after SCI, 28 day after SCI, respectively, 10 rats per group）. After the establishment of animal model of SCI, the functional recov- ery of the hind limb was evaluated by BBB score （The Basso, Beattie and Bresnahan locomotor rating scale） at 1 d,7 d,14 d, 21 d and 28 d. Then the rats were sacrificed to undergo HE staining and im- munohistochemical staining. The morphological change of spinal cord, and GFAP and MBP positive cells after SCI were observed through optical microscope. Results： In BBB score evaluation, the rats of SCI model groups paralyzed after anesthetic awareness. The neurologic function of the SCI rats＇hind limb began to recover gradually, with the recovery being most obvious at 14d. HE staining showed that 1 d after SCI operation, in injured area diffusing hemorrhage, neuronal degeneration and necrosis could be observed. At 7 d after SCI operation, inflammatory cell infiltration could be observed. Capsule cavity formed at 14 - 21 d after SCI operation. At 28 d after SCI operation, spinal cord injury was replaced by cicatricial tissue. Immunohistochemistry staining showed that the expression of GFAP posi- tive cells in SCI model groups increased compared with normal control group and sham group. The as- trocyte in the SCI area proliferated and hypertrophied, with hyperplasia being most obvious at 14 d af- ter SCI operation. The expression of MBP positive cells decreased obviously at 1 d after SCI operation. The myelin sheath in the SCI area was destroyed. At 7 d after SCI operation, the axon clearance began to widen, the expression of MBP positive cells began to increase. At 14 d after SCI operation, the axon clearance

关 键 词：脊髓损伤 神经胶质细胞 胶质纤维酸性蛋白 髓鞘碱性蛋白 大鼠 Sprague-Dawley 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]