通阳刮痧疗法对佐剂性关节炎大鼠TNF-α及T淋巴细胞亚群的影响  

Influence of Tong Yang Scraping Therapy on TNF-α,T Lymphocyte Subsets in Adjuvant Arthritis Rats

作　　者：苏荣华[1] 刘颖[1] 王光义[2] 吕明庄[2] 

机构地区：[1]贵州医科大学,贵州贵阳550004 [2]贵州医科大学附院针灸科,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第9期919-921,共3页Journal of Guiyang Medical College

基　　金：国家中管局全国中医药名老专家传承工作室建设项目(2014)

摘　　要：目的:探讨通阳刮痧疗法对佐剂性关节炎大鼠的治疗效果及可能机制。方法:将大鼠随机分为正常对照组、模型组、刮痧组,采用弗氏完全佐剂建立佐剂性关节炎大鼠模型(AA),采用足容积法检测大鼠足部肿胀度,酶联免疫法检测肿瘤坏死因子-α(TNF-α),流式细胞仪检测T淋巴细胞亚群(CD4+/CD8+)的比值。结果:模型组与正常组相比,大鼠足部肿胀明显、CD4+/CD8+比值下降,TNF-α含量升高,差异有统计学意义(P<0.05);刮痧组和模型组相比,大鼠足部肿胀减轻、CD4+/CD8+比值升高,TNF-a含量下降,差异有统计学意义(P<0.05);结论:通阳刮痧疗法对AA大鼠机体免疫功能有良性的调节作用,其机制可能是降低血清TNF-α含量,调整外周血T淋巴细胞亚群CD4+/CD8+的比值,使失衡的免疫功能得到恢复。Objective： To investigate the therapeutic effect of Tong Yang scraping therapy on adju- vant arthritis rats and its possible mechanism. Methods： The adjuvant arthritis rat model （AA） was established by Freund complete adjuvant, rats were randomly divided into normal control group, model group, scrapping group. The rat foot swelling was detected by the foot volume method, tumor necro- sis factor-α （TNF-α） was detected by enzyme-linked immunoassay （ELISA）, and T lymphocyte sub- sets （CD4＋/CD8＋ ） ratio was detected by Flow Cytometry. Results： Compared with normal control group, in model group rats＇feet swelled significantly, the ratio of CD4 ＋/CD8＋ decreased significantly, and levels of TNF-α increased significantly （ P 〈 0.05 ）. Compared with model group, in scrapping group the swelling levels of rats＇feet alleviated, levels of TNF-α were reduced, and CD4＋/CD8＋ ratio increased. And the differences were statistically significant （ P 〈 0.05 ）. Conclusion： Tong Yang scraping therapy on AA rats plays a positive regulation role in immune function. The mechanism may be that it can decrease the content of serum TNF-α, regulate the ratio of peripheral blood T lymphocyte subsets CD4＋/CD8＋ , and recover imbalanced immune function.

关 键 词：通阳刮痧疗法 佐剂性关节炎 肿瘤坏死因子-Α T淋巴细胞 大鼠 Sprague-Dawley 

分 类 号：R593.2[医药卫生—内科学]