川芎嗪对脓毒症大鼠肝细胞线粒体体视学的影响  

Effects of tetramethylpyrazine on stereology of hepatocyte mitochondria in septic rats

作　　者：王锦权 陶晓根[1,2] 张霖 刘海华[1,2] 姚秀英 张兴荣[1,2] 李坤 Li Wei[3] 黄业华 

机构地区：[1]安徽医科大学附属省立医院 [2]安徽省立医院南区重症医学科,合肥230001 [3]UCL School of Pharmacy,University College London [4]安徽省立医院肾内科,合肥230001

出　　处：《中国临床保健杂志》2015年第4期392-395,I0003,共5页Chinese Journal of Clinical Healthcare

基　　金：安徽省国际合作项目(1403062022)

摘　　要：目的研究川芎嗪(TMP)对脓毒症大鼠肝细胞线粒体结构的保护作用及可能机制。方法实验大鼠被分成对照组、脓毒症组、治疗组和预防组;用盲肠结扎穿孔法制备大鼠脓毒症实验模型,在术后10 h,取肝脏标本用JEM-1230透射电子显微镜观察肝细胞的超微结构,选用Image-Pro Plus 6.0图像软件对线粒体进行测量;用Western blot技术检测肝细胞线粒体内膜水通道蛋白(AQP)8的表达。结果和对照组比较,其余3组单个肝细胞切面的线粒体总数和数密度均减少(P<0.01),但治疗组和预防组均多于脓毒症组(P<0.01);线粒体体积密度脓毒症组也较对照组低(P<0.01);线粒体内膜AQP8的相对表达量在脓毒症组、治疗组和预防组均较对照组明显减少(P<0.01);治疗组和预防组的AQP8相对表达量较脓毒症组增多(P<0.01)。结论在脓毒症状态下,TMP可保护肝细胞线粒体的结构,可能是通过增加线粒体内膜AQP8表达而起作用的。Objective To study the protective effects and plausible mechanisms of tetramethylpyrazine （TMP）on the structure of hepatocyte mitochondria in septic rats.Methods The rats were randomly divided into four groups：control group,septic group,therapeutic group and prevention group.The model of septic rat was established by the cecal ligation and puncture（CLP）.The liver was taken out from septic rat 10 h after CLP,.The ultrastructure of hepatocyte were observed by the JEM-1230 transmission electron microscope.The hepatocyte mitochondria were meas-ured by Image-Pro Plus 6.0 analysis software.The expression of AQP8 was assayed by Western blot.Results Com-pared with the control group,the total number of mitochondria and numberical density were fewer in other 3 groups. The total number of mitochondria and numberical density of septic group were more than the therapeutic and preven-tion group（P〈0.01）,but the volume density of septic group was lower than the control group（P〈0.01）.Compared with the control group,the relative AQP8 protein expression was significantly reduced in the other 3 groups（P〈0.01）.The AQP8 protein expression of the therapeutic and prevention group were higher than the septic group（P〈0.01）.Conclusion TMP can protect stereology of hepatocyte mitochondria in septicrats by increasing the expression of AQP8 protein.

关 键 词：脓毒症 线粒体 肝 川芎嗪 大鼠 Sprague-Dawley 

分 类 号：R459.7[医药卫生—急诊医学]