祛风清热定痛方对偏头痛大鼠镇痛效应及内皮素1及5-羟色胺含量的影响  

Observation of Qufeng- qingre- dingtong decoction on Analgesia effects and the content of ET-1,5-HT in migraine rats

作　　者：李瑞杰 冀绪 白杏强 张晓双[3] 司宁宁 

机构地区：[1]河北省新乐市中医院中风病科,河北新乐050700 [2]河北省新乐市长寿街道办事处社区卫生服务中心,河北新乐050700 [3]陕西中医药大学药学院中药教研室,陕西咸阳712046 [4]河北省滦平县中医院内科,河北滦平068250

出　　处：《河北中医》2015年第8期1183-1186,共4页Hebei Journal of Traditional Chinese Medicine

基　　金：河北省中医药管理局2011年度中医药类科研计划课题(编号:2011141)

摘　　要：目的观察祛风清热定痛方对偏头痛大鼠的镇痛效应及可能机制。方法将72只SD大鼠随机分为6组,即空白组、模型组、正天丸组和祛风清热定痛方大、中、小剂量组,每组各12只,分别予相应药液灌胃给药,连续7 d。除空白组外,其余5组大鼠通过热板法测定痛阈值,然后以硝酸甘油制备偏头痛大鼠模型,放免法检测各组大鼠血浆中内皮素1(ET-1)、5-羟色胺(5-HT)含量。结果与模型组同期比较,祛风清热定痛方大、中剂量组60、120、180 min痛阈值均显著提高(P<0.05),祛风清热定痛方小剂量组、正天丸组120、180 min痛阈值均显著提高(P<0.05)。与模型组比较,祛风清热定痛方大、中、小剂量组及正天丸组ET-1含量均明显降低,5-HT含量均明显增加,比较差异有统计学意义(P<0.05)。与正天丸组比较,祛风清热定痛方大、中剂量组ET-1含量明显降低,5-HT含量明显增加,差异有统计学意义(P<0.05),小剂量组ET-1和5-HT含量无统计学意义(P>0.05)。与祛风清热定痛方大剂量组相比,祛风清热定痛方中剂量组ET-1和5-HT含量比较差异有统计学意义(P<0.05),小剂量组无统计学意义(P>0.05)。结论祛风清热定痛方具有明显的镇痛作用,其调节偏头痛模型大鼠血浆5-HT、ET-1含量可能是治疗偏头痛的机制之一。Objective To observe the therapeutic effects of Qufeng-qingre-dingtong decoction in migraine rats and its possible mechanism.Methods 72 SD rats were randomly divided into six groups, that the blank group, model group, Zhengtian pill group, and high, middle, low dose of Qufeng -qingre -dingtong decoction group. Twelve SD rats in each groups, and all rats received corresponding drug for 7 d by gavage.Besides blank group, rats in other five groups were constructed in migraine model by nitroglycerin after the pain-threshold measured by hot plate test.The contents of serum endothelin-1 （ET-1） and 5-hydroxytryptamine （5-HT） were detected by ra-dioimmunoassay.Results Compared with model group over the same period, the pain-threshold in high, middle dose of Qufeng-qingre-dingtong decoction group were obviously increased at 60, 120, 180 min （P〈0.05）, the pain-threshold in low dose of Qufeng-qingre-dingtong decoction group and Zhengtian pill group were obviously in-creased at 120, 180 min （P〈0.05）.Compared with model group, the content of ET-1 was obviously decreased in high, middle, low dose of Qufeng -qingre -dingtong decoction group and Zhengtian pill group, the differences were statistical significance （ P 〈0.05 ） . Compared with Zhengtian pill group, the content of ET-1 were obviously decreased in high, middle dose of Qufeng-qingre -dingtong decoction group, and the content of 5 -HT was obviously increased, the differ-ences were statistical significance （ P 〈0.05 ） .There was no statistical significance on ET-1 and 5-HT be-tween Zhengtian pill group and low dose group （ P 〉0.05）.Compared with high dose of Qufeng-qingre-dingtong decoction group, there was statistical significance on ET-1 and 5-HT in middle dose group （P〈0.05）, and there was no statistical significance in low dose group （ P 〉0.05）.Conclusion Qufeng-qingre-dingtong decoction has significant analgesic effect, can regulate the contents of serum 5-HT, ET-1 in migraine rats, it could be the one of the analges

关 键 词：偏头痛 祛风 清热 镇痛 中医药疗法 止痛剂(中药) 放射免疫测定 内皮缩血管肽类 血清素 疾病模型 动物 Paregoric ( Chinese herbs) 

分 类 号：R332[医药卫生—人体生理学] R287[医药卫生—基础医学]