糖皮质激素性骨质疏松症大鼠模型制备及中药的干预作用  

Study of Rat Model with Glucocorticoid-induced Osteoporosis and Effects of Chinese Herbs

作　　者：朱辉[1] 郑洪新[1] 林庶如[1] 杨芳[1] 王剑[1] 张国哲[1] 

机构地区：[1]辽宁中医药大学基础医学院,辽宁沈阳110847

出　　处：《辽宁中医药大学学报》2015年第8期30-32,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁省科技厅博士启动基金项目(20121102);辽宁省教育厅一般项目(L2012352)

摘　　要：目的:建立糖皮质激素性骨质疏松症(GIO)大鼠模型,观察不同中药的防治作用。方法:Wistar大鼠132只(雌雄各半),随机分为正常组、模型组、补肾组、健脾组、活血组及骨疏康组。除正常组外,其余各组给予地塞米松(2.5 mg/kg)肌注,每周2次。除正常组及模型组外,其余各组予以相应的中药治疗。造模及给药连续9周。9周后,测定离体股骨骨密度(BMD)及血清骨代谢相关指标。结果:与正常组比较,模型组大鼠BMD下降(P<0.01),血清抗酒石酸酸性磷酸酶(TRAP)的含量升高(P<0.01)。相对于模型组,各治疗组BMD表现出不同程度的升高,其中补肾组升高程度最显著(P<0.01);各治疗组血清TRAP含量显著降低,均有统计学意义。结论:地塞米松诱导的GIO大鼠模型制备成功。补肾中药对GIO具有一定的拮抗作用。Objective ： To replicate the rat model with GIO, and to observe the preventive and therapeutic effects of different Chinese herbs. Methods ： 132 Wistar rats male and female half and half were randomly divided into normal group, model group, nourishing-kidney group, strengthening-spleen group, activating- blood group and GUSHUKANG group. By intramuscular injection of dexamethasone （ 2.5 mg · kg-1 ） twice a week to replicate osteoporosis rat model, to follow with treatment by different Chinese herbs except normal and model groups. These process were lasted for 9 weeks. After 9 weeks, detected the BMD of femur in vitro and the bone metabolism marker in serum. Results ： Compared with normal group, the BMD of model group decreased obviously （P〈0.01）, the content of TRAP in serum increased evidently （P〈0.01）. Compared with model group, the BMD of each Chinese herbs treatment group up-regulated, nourishing-kidney group were most significant （P〈0.01）. The content of TRAP in serum of each Chinese herbs treatment group down-regulated, the differences had statistical significance. Conclusion ： Intramuscular injection of dexamethasone can induce GIO rat model, nourishing-kidney herbs have the effect of anti-osteoporosis.

关 键 词：糖皮质激素性骨质疏松症 动物模型 地塞米松 中药治疗 

分 类 号：R714.257[医药卫生—妇产科学]