高效液相色谱法测定复方丹参片与滴丸中7种活性成分含量  

Determination of Seven Active Components Compared with Fufang Danshen Tablets and Dripping Pills by HPLC

作　　者：张爱兵 张珺[2] 程月发[2] 张春雨 郭兰[2] 刘颖硕[2] 

机构地区：[1]河北省唐山市食品药品检验中心化学室,唐山063000 [2]河北联合大学冀唐学院,唐山063300

出　　处：《医药导报》2015年第8期1067-1071,共5页Herald of Medicine

基　　金：唐山市2013年科学技术研究与发展计划基金资助项目(13130298z);2012年河北联合大学科学研究基金资助项目(z201242)

摘　　要：目的建立高效液相色谱（HPLC）法同时测定复方丹参片和复方丹参滴丸中7种活性成分的含量。方法采用Zorbax XDB-C18色谱柱,以乙腈-0.1%磷酸溶液为流动相,梯度洗脱,柱温30℃,流速1 m L·min^-1,检测波长为203,270和281 nm。结果 7种成分峰面积与浓度的线性关系良好;加样回收率在95.1%~100.4%;滴丸样品中丹参酮ⅡA未能检出。结论建立了同时测定两类复方丹参制剂中丹参素、原儿茶醛、三七皂苷R1、人参皂苷Rg1、丹酚酸B、人参皂苷Rb1和丹参酮ⅡA含量的方法,精密度高,重复性好,可用于两类复方丹参制剂的质量控制;复方丹参片中丹参酮ⅡA含量明显高于滴丸。Objective To establish a HPLC method for simultaneous determination of seven active components in Fufang Danshen tablets and Fufang Danshen dripping pills. Methods These seven compounds were analyzed simultaneously with a Zorbax C18column by gradient elution using acetonitrile-0.1% phosphoric acid solution as mobile phase, the flow rate was 1 mL · min^-1 and the detection wavelength was set at 203, 270 and 281 nm, respectively. Results All the seven components showed good linear relation between peak area and concentration of the test, and the average recoveries were between 95.1%- 100.4%.Tanshinone Ⅱ A was not detected in samples of dropping pills. Conclusion The HPLC method to determine the components including tanshinone II A, salvianolic acid B, propanoid acid, protocatechuic aldehyde, notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 of the two different Danshen preparations has been established, and it has the advantages of simplicity, high precision, good repeatability, and can be used for the quality control of two kinds of Fufang Danshen preparations.The content of tanshinone Ⅱ A in Fufang Danshen tablet was distinctly higher than that of dropping pills.

关 键 词：丹参片 复方 丹参滴丸 复方 活性成分 色谱法 高效液相 

分 类 号：R286[医药卫生—中药学] R927.2[医药卫生—中医学]