断眼天牛DNA条码试剂盒的研制  

DNA barcoding identification kit technology for Tetropium species

作　　者：陈梦义[1,2] 徐梅[3] 钱路[3] 安榆林[3] 王焱 郝德君[1,2] 

机构地区：[1]南京林业大学南方现代林业协同创新中心,南京210037 [2]南京林业大学林学院,南京210037 [3]江苏出入境检验检疫局,南京210001 [4]上海市林业总站,上海200072

出　　处：《应用昆虫学报》2015年第4期854-861,共8页Chinese Journal of Applied Entomology

基　　金：国家科技支撑计划课题(2012BAK11B03);上海市绿化管理局2011年科研项目(JB119927);江苏省高校优势学科建设工程资助项目(PAPD)

摘　　要：【目的】为了加强对检疫口岸截获木材中的断眼天牛种类进行准确、快速的鉴定,克服单纯依赖于形态学方法为主的局限性,我们研发了断眼天牛属DNA条码试剂盒检测技术。【方法】本研究基于线粒体COⅠ基因(线粒体细胞色素氧化酶亚基Ⅰ),采用加拿大条码中心开发的昆虫及植物DNA提取方法,对基因序列分析,获得碱基位点规律。【结果】断眼天牛属中的9个常截获种类取得了标记性碱基位点。【结论】检疫中常截获的断眼天牛属中的9个种通过标记性碱基位点得到区分;研发了断眼天牛属DNA条码试剂盒检测技术,为进出境口岸的检疫工作提供一种新的鉴定手段。[Objectives] To rapidly and accurately identify Tetropium spp., which are invasive pests commonly intercepted at quarantine ports in shipments of wood, and improve on current, mainly morphologically-based, methods of identifying these pests. [Methods] A DNA barcode detection kit was developed for Tetropium spp. based on the mitochondrial CO I gene. DNA extraction methods followed those of the Canadian Plant Development DNA barcode Center. [Results] Nine base sites were obtained from Tetropium spp. [Conclusion] Analysis of different genetic characteristics among Tetropium spp. allowed nine species to be identified. The newly developed DNA barcoding identification kit provides a new way of identifying this pest at oorts of entrv and exit.

关 键 词：断眼天牛属 DNA条形码 CO I基因 分子鉴定 

分 类 号：S763.38[农业科学—森林保护学]