几种无机离子和有机小分子对黄孢原毛平革菌的酶活性影响分析  

Impact of Inorganic Ions and Organic Small Molecules on Enzymatic Activity of Phanerochaete chrysosporium

作　　者：陈英[1] 杨伟球[1] 邱学林[1] 林惠菲 

机构地区：[1]苏州农业职业技术学院生态环境系,江苏苏州215008

出　　处：《湖北农业科学》2015年第12期2878-2881,共4页Hubei Agricultural Sciences

基　　金：苏州农业职业技术学院2012年度科研创新团队建设项目(ITM201205)

摘　　要：以黄孢原毛平革菌(Phanerochaete chrysosporium)为代表的白腐真菌,其分泌的木质素过氧化物酶(Li P)和锰过氧化物酶(Mn P)等酶系能降解木质素以及前体类似物。对几种无机金属离子、无机阴离子、有机小分子对黄孢原毛平革菌所产酶系中的木质素过氧化物酶(Li P)和锰过氧化物酶(Mn P)的酶活性影响进行了分析。结果表明,Fe3+对木质素过氧化物酶(Li P)和锰过氧化物酶(Mn P)都有很强的抑制作用,Fe2+、Ca2+对木质素过氧化物酶(Li P)和锰过氧化物酶(Mn P)表现出不同的促进作用,Br-比Cl-的激活作用稍弱;在添加乙二胺四乙酸(EDTA)或抗坏血酸的条件下,限氮培养比富氮培养更能提高出发菌株和诱变菌株的酶活力。As the representative of White-rot Fungus,Phanerochaete chrysosporium can secrete Lignin peroxides and Manganese peroxides and so on,which can depredate Lignin and Precursor analogs. Through using Inorganic ions and Organic molecules as Activator, enzyme activity in different conditions was researched.The results show that Fe^3＋on LiP and MnP had strong inhibitory effect,but Fe^2＋and Ca^2＋on those showed different promoting effect. Br^-can improve the enzyme activity better than Cl^-. In addition of EDTA or ascorbic acid conditions,the nitrogen limited culture than nitrogen rich culture can improve the enzyme activity of the strains.

关 键 词：黄孢原毛平革菌(Phanerochaete chrysosporium) 木质素过氧化物酶 锰过氧化物酶 酶活力 

分 类 号：O629.8[理学—有机化学]