菜豆植物凝集素活性的测定方法研究  

Determination of the Activity of Lectin in Kidney Bean

作　　者：李佳楠[1] 杨薇[1] 袁莎[1] 陈禅友[1] 

机构地区：[1]江汉大学生命科学学院生物技术系,武汉430056

出　　处：《湖北农业科学》2015年第12期3005-3007,共3页Hubei Agricultural Sciences

基　　金：武汉市青年晨光计划项目(2013071004010476);武汉市科技攻关项目(201250499145-11;2013071004010478)

摘　　要：探索分光光度法测定菜豆(Phaseolus vulgaris)植物凝集素活性的最佳测定条件,分析了56个菜豆品种的植物凝集素活性。结果表明,红细胞悬液浓度2×108个/m L,凝血10 min后测定OD416 nm,为菜豆植物凝集素的最佳测定条件。在该条件下,分析了56个菜豆品种中的植物凝集素活性浓度,旨在为分析菜豆植物凝集素的毒效提供参考。The optimal detecting lectin activity in Phaseolus vulgaris by spectrophotometry and 56 varieties were analyzed in this paper. The results showed that the suitable concentration of red blood cell was 2×108cell / mL, and the best coagulation time was 10 min and detected at 416 nm. The lectin activity concentration of 56 varieties were determined under to the established conditions to provide a reference for the toxic effects analysis of lectin in Phaseolus vulgaris.

关 键 词：菜豆(Phaseolus vulgaris) 植物凝集素 分光光度法 活性 

分 类 号：S532[农业科学—作物学]