植物耐盐基因筛选方法及其转基因育种研究  

Screening Method of Salt Tolerance Genes and Transgenic Breeding in Plants

作　　者：韩强[1,2] 贺雪莲[1,2] 卓仁英[2] 何正权[1] 

机构地区：[1]三峡大学生物技术研究中心,湖北宜昌443002 [2]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《湖北农业科学》2015年第13期3078-3082,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31300508)

摘　　要：植物对盐胁迫的耐受性是一个复杂的过程,涉及渗透调节、离子平衡和区域化及抗氧化防御系统等多个方面。综述了植物耐盐基因的筛选方法(抗性表达文库、耐盐突变体的图位克隆、基因差异表达和电子克隆)及通过这些方法所获得的部分耐盐基因;并在此基础上简述了耐盐基因在改良植物抵御盐胁迫方面的应用概况。Salt stress tolerance of plant is a complex process involving in osmotic adjustment, ion balance and regionalization and antioxidant defense system. The screening method for plant salt tolerance genes （resistance expression library construction, Map-based cloning of sah-tolerant mutant, differentially expressed genes and electronic cloning） and some salt tolerance genes obtained by these methods were reviewed, and based on this, the general situation of the application of salt-tolerant genes improving plants salt stress tolerance was briefly discussed.

关 键 词：盐胁迫 耐盐基因 基因克隆 转基因育种 

分 类 号：Q789[生物学—分子生物学]