不同颜色蚕豆的红外光谱分析及元素含量测定  

Infrared Spectrum Analysis and Elements Determination of Broad Bean of Different Colors

作　　者：徐娟[1] 刘刚[1] 欧全宏[1] 赵帅群 任静[1] 胡见飞 

机构地区：[1]云南师范大学物理与电子信息学院,昆明650500

出　　处：《湖北农业科学》2015年第13期3244-3247,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30960179)

摘　　要：利用傅里叶变换红外光谱（FTIR）和电感耦合等离子体质谱（ICP-MS）对白色蚕豆和绿色蚕豆进行研究。结果显示，蚕豆子叶的红外光谱主要由蛋白质、多糖等振动吸收谱带组成。两种蚕豆的红外光谱相似，但在一些特征峰上的吸光度比值存在差异。对1700～1600cm。波段内的原始光谱进行拟合．绿色蚕豆的蛋白质二级结构中α螺旋、β转角和无规则卷曲结构的含量均比白色蚕豆高，而 β折叠含量低于白色蚕豆。ICP-MS测试结果表明，蚕豆中富含P、K、Na、Ca、Mg、Cu、Fe、Zn、Mn等多种矿质元素，其中元素P、K的含量最高，Ca、Mg、Na、Fe、Zn和Mn含量丰富；Cu含量相对较低。其中，白色蚕豆的矿质元素K、Ca、Cu和Na含量比绿色蚕豆略高，而其他矿质元素P、Mg、Fe、Zn,Mn含量低于绿色蚕豆。研究表明，FTIR结合ICP-MS方法可以快速简便区分两种蚕豆。Fourier transform infrared spectroscopy （FTIR） and inductively coupled plasma-mass spectrometry （ICP-MS） were used to study two kinds of broad bean. The FTIR results showed that the infrared spectra of broad bean were mainly com- posed of the absorption bands of protein and polysaccharide. The spectrum of samples Were similar,only with minor differences in absorbance ratios of several peaks. The amide I band （1 700-1 600cm^-1） in the original spectra was used for curve fitting to evaluate the contents of secondary structure of protein. The results showed the contents of α-helix, β-turn and unordered structure in green beans were higher than that in white beans,while the content of β-sheet in green beans less than that in white beans. ICP-MS results showed that the P,K,Ca,Mg,Cu,Fe,Zn and Mn were found in the broad bean,and the contents of P,K were the highest, followed by Ca,Mg,Na,Fe,Zn and Mn,and the lowest was Cu. The contents of K,Ca,Cu and Na in white beans were slightly higher than that in green beans,while the contents of P,Mg,Fe,Zn and Mn in white beans less than that in green beans. It was proved that FTIR spectroscopy combined with ICP-MS could be used to discriminate two kinds of broad bean fast and accurately.

关 键 词：蚕豆 傅里叶变换红外光谱 电感耦合等离子体质谱 元素 

分 类 号：O657.3[理学—分析化学]