软坚护肝片对刀豆蛋白A所致小鼠免疫性肝损伤的保护作用  

Protective effect of Ruanjian Hugan Tablet on immunological hepatic injury induced by ConA in mice

作　　者：莫凤珍[1] 孔晓龙[1] 

机构地区：[1]广西医科大学药学院,南宁市530021

出　　处：《广西医学》2015年第6期752-754,共3页Guangxi Medical Journal

基　　金：广西科学研究与技术开发计划项目(桂科攻10124008-7)

摘　　要：目的观察软坚护肝片(RJHGT)对刀豆蛋白A(Con A)所致小鼠免疫性肝损伤的保护作用,并探讨其作用机制。方法将60只小鼠随机分为正常组、模型组、阳性对照组、RJHGTH组、RJHGTM组、RJHGTL组,每组10只;正常组和模型组以0.2 ml/10 g生理盐水灌胃,阳性对照组给予联苯双酯0.15 g/kg灌胃,RJHGTH组、RJHGTM组、RJHGTL组以0.2 ml/10 g分别给予RJHGT溶液1.24、0.62、0.31 g/kg灌胃,1次/d,连续7 d。末次给药后4 h,正常组尾静脉注射生理盐水,其余各组尾静脉注射Con A制备小鼠免疫性肝损伤模型。检测各组小鼠血清肿瘤坏死因子α(TNF-α)和γ干扰素(IFN-γ)、ALT、AST等水平;检测肝组织谷胱甘肽(GSH)及超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)等指标。结果与模型组比较,各浓度RJHGT组小鼠血清ALT、AST、TNF-α、IFN-γ水平及肝组织中MDA、NO含量均降低,肝组织SOD和GSH水平均升高(P<0.05)。结论 RJHGT对免疫性肝损伤小鼠具有一定的保护作用。Objective To observe the protective effect of Ruanjian Hugan Tablet（ RJHGT） on immunological hepatic injury induced by ConA in mice,and to explore its mechanism.Methods Sixty mice were randomly divided into normal control group,model group,positive control group,RJHGTH group,RJHGTM group and RJHGTL group,with 10 mice in each group.Normal group and model group were given normal saline（0.2 ml/10 g） by gavage,positive control group was given bifendate（0.15 g/kg） by gavage,RJHGTH group,RJHGTM group and RJHGTL group were given RJHGT solution （0.2 ml/10 g） by gavage at the dosage of 1.24,0.62 and 0.31 g/kg,respectively,once a day for seven days.Four hours after the final administration,normal group was given venacaudalis injection with normal saline,while the other groups were given venacaudalis injection with ConA to develop the mouse model of immunological hepatic injury.The levels of serum tumor necrosis factor（TNF）-α,interferon（IFN）-γ,ALT and AST of the mice were detected in each group,and the contents of glutathione（GSH）,superoxide dismutase（SOD）,malondialdehyde（MDA） and nitric oxide（NO） in hepatic tissues were detected as well.Results Compared with model group,the mice in each RJHGT group showed decreased levels of serum ALT,AST,TNF-αand IFN-γ,decreased contents of MDA and NO in hepatic tissues,and increased levels of SOD and GSH in hepatic tissues （P 〈0.05）. Conclusion RJHGT has protective effects on mice with immunological hepatic injury.

关 键 词：软坚护肝片 免疫性肝损伤 脂质过氧化 小鼠 

分 类 号：R285.5[医药卫生—中药学]