广州市番禺地区A2亚型频率调查  

Survey on frequency of A2 subgroup in Panyu Prefecture,Guangzhou

作　　者：莫锦政 谢敬文 蓝文莉 陈结贞 雷名禄 

机构地区：[1]广东省广州市番禺区中心血站,广州市511400 [2]广州市番禺区市桥医院,广州市511400 [3]广州市南沙区第二人民医院,广州市511455

出　　处：《广西医学》2015年第6期810-812,共3页Guangxi Medical Journal

基　　金：广东省番禺区科技计划医药卫生项目(2013-Z03-64)

摘　　要：目的分析A2亚型频率分布和基因分型的特征。方法选择广州市番禺地区户籍参加献血和在医院体检人员6 640例,其中A型血5 262例,AB型血1 378例;采用120孔微量板法,使用抗-A1试剂对血样本进行抗-A1筛查;采用试管法,对抗-A1阴性样本进行血清学A2亚型表型鉴定。采用序列特异性引物-聚合酶链反应(PCR-SSP)法(A2基因分型检测试剂盒)对抗-A1阴性样本的基因组DNA进行扩增,对扩增产物进行电泳分析,根据电泳图谱判断A2基因分型类型。结果在5 262例A型血样本中,筛查出抗-A1阴性14例,经鉴定均为A2亚型,发生频率为0.27%(14/5 262),其中男性10例,女性4例。在1 378例AB型样本中,筛查出抗-A1阴性48例,经鉴定均为A2B亚型,发生频率为3.48%(48/1 378),其中男性34例,女性14例。在14例A2样本中,检出A201基因型2例,A205基因型4例,发生频率为0.11%(6/5 262);在48例A2B样本中,检出A201基因型4例,A205基因型15例,发生频率为1.38%(19/1 378)。结论广州番禺区A2基因亚型以A205为主,使用血清学结合PCR-SSP方法能准确鉴定A2亚型。Objective To analyze the frequency distribution and genotyping characteristics of A2 subgroup.Methods 6 640 cases participated in blood donation and clinical examination were enrolled in the study,including 5 262 cases of A blood group and 1 378 cases of AB blood group.Their household register was Panyu Prefecture,Guangzhou City.The 120-hole micro-plate method was applied to anti-A1 screening in blood samples with anti-A1 reagent.The test tube method was used to perform serological A2 subgroup phenotypic identification in negative anti-A1 samples.Polymerase chain reaction-sequence specific primers（PCR-SSP） with A2 genotyping assay kit was used to amplify the genome DNA of negative anti-A1 samples,and then electrophoresis was adopted to analyze the amplified products. A2 genotype was determined based on the electrophorograms.Results Fourteen negative anti-A1 cases（10 male,4 female） were screened out from 5 262 samples of A blood group,identified as A2 subgroup,the negative frequency was 0.27%（14 /5 262）.Forty-eight negative anti-A1 cases（34 male,14 female） were screened out from 1 378 samples of AB blood group,identified as A2B subgroup,the negative frequency was 3.48%（48 /1 378）.There were 2 A201 cases and 4 A205 cases among 14 A2 samples with the frequency of 0.11%（6/5 262）. There were 4 A201 cases and 15 A205 cases among 48 A2B samples with the frequency of 1.38%（19 /1 378）.Conclusion A2 subgroup is dominated by A205 in Panyu Prefecture, Guangzhou City.A2 subgroup can be accurately identified with serological method and PCR-SSP.

关 键 词：血型 A2 亚型 A2B 亚型 序列特异性引物 -聚合酶链反应 基因分型 

分 类 号：R457.11[医药卫生—治疗学]