ADD1基因在贵州地方山羊组织器官中和品种间的表达差异比较  

Comparison of ADD1 gene expression in tissues and among breeds of Guizhou native goat

作　　者：杨家大[1] 

机构地区：[1]凯里学院环境与生命科学学院,贵州凯里556011

出　　处：《浙江农业学报》2015年第7期1134-1140,共7页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金项目(81460572);贵州省科技联合基金项目[黔科合J字LKK(2013)14];凯里学院规划课题(Z1304);凯里学院获得博士学位(引进教授)教师专项(BS201303);贵州省普通本科高校自然科学研究项目[黔教合KY字(2014)236]

摘　　要：以黔东南小香羊、贵州白山羊、贵州黑山羊、黔北麻羊、南江黄羊为研究对象,采用实时荧光定量技术对肝、肾、心、肺、背最长肌、半膜肌、皮下脂肪中ADD1基因的表达水平进行测定。结果表明,黔东南小香羊ADD1基因的表达水平无组织器官差异(P>0.05),贵州白山羊表达水平以背最长肌、心和半膜肌较高(P<0.05),贵州黑山羊肝内较高,黔北麻羊肾内较高,南江黄羊心内较高。心和半膜肌中ADD1基因的表达无品种差异(P>0.05),肝、肾、肺、背最长肌和皮下脂肪中ADD1基因的表达存在品种差异(P<0.05)。结果提示,贵州地方山羊ADD1基因的组织器官表达模式不一致,且其组织器官表达水平可能存在品种差异。Expression levels of ADD1 gene in livers, kidneys, hearts, lungs, longissimus muscle, semimembranosus muscle and subcutaneous fat of Qiandongnan small Xiang goat, Guizhou white goat, Guizhou black goat, Qianbei Ma goat and Nanjiang yellow goat were detected by real-time fluorescence quantitative RT-PCR. The results showed that, there were no differences of the expression levels in tissues of Qiandongnan small Xiang goat（ P 〉 0. 05 ）. High ex- pression levels were observed in longissimus muscle, heart and semimembranosus muscle of Guizhou white goat（ P 〈 0. 05 ） , liver of Guizhou black goat, kidney of Qianbei Ma goat, and heart of Nanjiang yellow goat. In hearts and semimembranosus muscle, there were no differences of expression levels of ADD1 gene among breeds （ P 〉 0.05 ）. But, in livers, kidneys, lungs, longissimus muscle and subcutaneous fat, significant differences of ADD1 expression level were detected（P 〈 0. 05 ）. The results suggested that the tissue expression patterns and levels of ADD1 gene in Guizhou native goat breeds were different.

关 键 词：贵州地方山羊 ADD1 基因表达 

分 类 号：S827[农业科学—畜牧学]