水蒸气蒸馏与乙醇提取化橘红叶成分的GC-MS分析  

GC-MS Analysis of the Extracts by Steam Distillation and Ethanol Extraction from Citrus Grandis Leaves

作　　者：韩寒冰[1] 王明阳 刘杰凤[1] 马超[1] 李海航[3] 

机构地区：[1]广东石油化工学院化学与生命科学学院,广东茂名525000 [2]茂名出入境检验检疫局,广东茂名525000 [3]华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广东广州510631

出　　处：《食品研究与开发》2015年第11期117-119,136,共4页Food Research and Development

基　　金：广东省科技计划项目(2013B020503069)

摘　　要：分析比较水蒸汽蒸馏法与乙醇提取化橘红叶化学成分的差异。通过水蒸汽蒸馏提取和无水乙醇超声辅助提取化橘红叶中的化学成分,运用GC-MS技术对其成分进行分离鉴定,用峰面积归一化法进行定量分析。水蒸汽蒸馏提取法共分离出58种成分,鉴定出其中的18种,主要成分是:β-蒎烯(19.44%)、β-月桂烯(6.95%)、D-柠檬烯(10.49%)、γ-萜品烯(19.30%)和石竹烯(20.46%)等;乙醇提取法共分离出80种成分,鉴定出其中的43种,主要成分是亚油酸(16.8%)、硬脂酸(6.57%)、菜油甾醇(5.82%)、豆甾烯醇(7.12%)和扶桑甾醇氧化物(14.28%)等,同时鉴定了甲氧基香豆素、补骨脂素、佛手柑内酯、前胡内酯和橙皮油内酯等香豆素类化合物,以及柚皮素、角鲨烯和VE等活性成分。两种不同的提取法共分离鉴定了18种相同的化学成分,乙醇提取法多分离鉴定25种成分。To analyse the chemical constituent of the extracts from leaves of Exocarpium Citrus Grandis by different extraction methods. The compounds were extracted by steam distillation and ethanol separately , constituents were separated and identified by GC-MS , and were quantified by the accuracy of area percent. 58 components were separated , among which 18 were identified, the main components wereβ-Pinene （19.44%）,β-Myrcene （6.95%）, Limonene （10.49%）,γ-Terpinene （19.30%） and Caryophyllene （20.46%） by steam distillation. 80 components were separated , among which 43 were identified , the main components were fatty acids （23.27%） and sterols （27.22%）, the main active ingredients were five cumarins （Herniarin, Ficusin, Bergapte, Ammidin and Aurapten）, Naringenin, Supraene and Vitamin E in the alcohol extraction. 18 chemical components of the extracts were the same by the two extraction methods, but there were more than 25 components in the alcohol extraction.

关 键 词：GC-MS 化橘红叶 水蒸气蒸馏法 乙醇提取法 

分 类 号：TQ914[化学工程]