异甘草酸镁对奥沙利铂在人胃癌裸鼠模型中抗肿瘤作用的影响研究  

Effects of magnesium isoglycyrrhizinate on oxalipatin chemotherapy in subcutaneously transplanted tumor of human gastric in nude mice

作　　者：郭曼[1] 赵园园[1] 何信佳[1] 于丽[1] 张蕊[2] 叶宝玲[1] 

机构地区：[1]青岛大学附属医院肿瘤科,山东青岛266000 [2]青岛大学附属医院检验科,山东青岛266000

出　　处：《川北医学院学报》2015年第4期429-434,共6页Journal of North Sichuan Medical College

基　　金：中国高校医学期刊临床专项资金(11520574)

摘　　要：目的:探讨异甘草酸镁对奥沙利铂在人胃癌裸鼠模型中抗肿瘤作用的影响。方法:建立人胃癌SGC-7901细胞裸鼠皮下移植瘤模型,并随机分为高、中、低剂量异甘草酸镁(Mg IG)联合奥沙利铂(L-OHP)组,单纯L-OHP组,单纯Mg IG组和葡萄糖对照组6组,连续尾静脉注射给药14 d,停药24 h后处死裸鼠,称取移植瘤质量,测量移植瘤体积,分别计算质量抑瘤率、体积抑瘤率,用TENEL法检测移植瘤组织的细胞凋亡率,并检测裸鼠肝功等指标。结果:高、中、低剂量Mg IG联合L-OHP组以及单纯L-OHP组的体积抑瘤率和质量抑瘤率均高于葡萄糖对照组,差异有统计学意义(P<0.05);各剂量Mg IG联合L-OHP组的抑瘤率高于单纯L-OHP组,且呈剂量依赖性,差异有统计学意义(P<0.05);与单纯L-OHP组相比,不同剂量Mg IG联合L-OHP组ALT和AST水平均较低,差异有统计学意义(P<0.01)。结论:异甘草酸镁对奥沙利铂的抗肿瘤作用具有协同作用,并能够潜在改善药物性肝损伤。Objective：To explore the effect of magnesium isoglycyrrhizinate on oxalipatin chemotherapy in subcutaneously trans-planted tumor of human gastric in nude mice. Methods：A tumor model of human gastric carcinoma was created by subcutaneous inocu-lation of SGC-7901 cells into nude mice. The mice were randomly assigned to high-,medium-,low-dose magnesium isoglycyrrhizinate with oxaliplatin groups,oxaliplatin group,magnesium isoglycyrrhizinate group and glucose control group with tail intravenous injection of drugs for 14 days. The mice were sacrificed 24 hours after the last injection and tissue specimens were obtained. Weights and volumes of the tumors were detected. Tumor inhibition rate was calculated and liver functions were tested. Results：The anti-tumor rates in high-, medium-,and low-dose magnesium isoglycyrrhizinate with oxaliplatin groups and oxaliplatin group were significantly higher than that in the glucose control group（P〈0. 05）. The anti-tumor rates of high-,medium-,and low-dose magnesium isoglycyrrhizinate with oxalipla-tin groups were higher than that of oxaliplatin group（P〈0. 05）,which showed a dose dependent manner. Compared with oxaliplatin group,high-,medium-,and low-dose magnesium isoglycyrrhizinate with oxaliplatin groups received a lower level of AST and ALT（ P〈0. 01）. Conclusion：Magnesium isoglycyrrhizinate has the potential to increase the anti-tumor effect of oxaliplatin,and shows an obvious protection of liver function.

关 键 词：异甘草酸镁 奥沙利铂 胃癌 裸鼠模型 肝损伤 

分 类 号：R318[医药卫生—生物医学工程]