毛细管电泳分析生物样本中儿茶酚氧甲基转移酶COMT活性  

Analysis of the activity of catechol-O- methyltransferase(COMT) in biological samples by capillary electrophoresis

作　　者：毛师师[1] 钦晓峰[1] 周杏琴[1] 

机构地区：[1]江苏省原子医学研究所卫生部核医学重点实验室江苏省分子核医学重点实验室,无锡214063

出　　处：《分析试验室》2015年第8期874-877,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(81371590);江苏省自然科学基金(BK2011167)资助

摘　　要：采用毛细管电泳快速检测生物样本中儿茶酚氧甲基转移酶（COMT）的活性。运行缓冲液为60%200 mmol/L PBS＋40%75 mmol/L硼酸（含0.05%三氟乙酸）,p H 10。在25~200μg/m L范围内线性关系良好,回归方程为Y=1.66×105x＋1.17×104,相关系数r2=0.9951。检出限0.02~0.5μmol/L（S/N≥3）。分析了抑郁症模型鼠以及叶酸和不同剂量PQQ腹腔注射后脑组织COM T活性的变化,为抑郁症的发病机制和药物作用靶点提供了新的依据。In this study,the activity of catechol-O-methyltransferase（ COMT） in biological samples was detected rapidly by capillary electrophoresis. Running buffer is 60% 200 mmol/L PBS and 40% 75 mmol/L boric acid（ with 0. 05% trifluoroacetic acid）,p H 10. Excellent linear correlation was showed in the range of 25 ~200 μg/m L.The regression equation was Y = 1. 66 × 105 x ＋ 1. 17 × 104 with the correlation coefficient r2= 0. 9951. The minimum detectable concentration of COMT was 0. 02 ~ 0. 5 μmol/L（ S/N≥3）. The change of COMT activity in brain tissues of depression model rats and rats with intraperitoneal injection of folic acid and different doses of PQQ was investigated. A new basis for the pathogenesis and drug targets of depression could be provided.

关 键 词：毛细管电泳 儿茶酚氧甲基转移酶COMT 抑郁症大鼠 脑组织 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.8[化学工程—制药化工]