液相色谱法同时检测胍基乙酸、氨基乙酸和单氰胺  

Simultaneous determination of guanidineacetic acid,glycine and aminonitrile by high performance liquid chromatography

作　　者：李秀瑜[1] 韩康[1] 韩亚楠[1] 段玉丰[2] 

机构地区：[1]河北师范大学分析测试中心,石家庄050024 [2]河北科技大学材料科学与工程学院,石家庄050018

出　　处：《分析试验室》2015年第8期886-890,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(31276272);河北省教育厅自然科学基金(Z2008118);河北师范大学自然科学基金(L2008Y11)项目资助

摘　　要：建立了同时检测胍基乙酸（GAA）、氨基乙酸（GLY）和单氰胺（AN）的高效液相色谱方法。样品经ODS（C18）柱分离,流动相0.04 mol/L KH2PO4/乙腈=95/5（V/V）洗脱,208 nm检测,在柱温30℃、流速0.6 m L/min时,12 min内完成检测。在考察范围内,色谱峰面积与浓度呈良好线性关系,相关系数在0.9972~0.9998之间,相对标准偏差（RSD,n=6）为0.93%~1.2%,回收率在97.9%~101%之间,GAA,GLY和AN的检出限分别为20.8,314,9.50 ng/m L。方法可满足胍基乙酸的产品质量控制、生产过程监控以及其它用途的检测需求。A method was established for the simultaneous determination of guanidine acetic acid（ GAA）,glycine（ GLY） and cyanamide（ AN） by high performance liquid chromatography（ HPLC）. The samples were separated by ODS（ C18） column,and detected by UV detector at 208 nm at 30°C in the mobile phase buffer of0. 04 mol/L potassium dihydrogen phosphate/acetonitrile = 95 /5（ V/V） at a speed of 0. 6 m L/min. The mixtures of GAA,GLY and AN were separated and determined in 12 minutes. In a certain range,the peak areas had good linear relationships with the concentrations of the compounds and their correlation coefficients were between 0. 9972- 0. 9998. The low limits of detection of GAA,GLY and AN（ S/N = 3） were 20. 8,314,and9. 50 ng/m L,respectively,in which the recoveries of GAA,GLY and AN were among 97. 9% ~ 101% with the relative standard deviations（ RSDs,n = 6） within 0. 93% ~ 1. 2%. The method is sensitive,reliable,and can meet requirements both in inspection quality control of guanidineacetic acid and its production process monitoring.

关 键 词：液相色谱 胍基乙酸 氨基乙酸 单氰胺 紫外检测 

分 类 号：TQ460.7[化学工程—制药化工]