纳米金比色法测定阿米卡星  

Determination of amikacin based on gold nanoparticles by colorimetric method

作　　者：田锐[1] 马晨[1] 王靖原[1] 李云云[1] 孙雪花[1] 韩学飞[1] 

机构地区：[1]延安大学化学与化工学院,延安716000

出　　处：《分析试验室》2015年第8期947-949,共3页Chinese Journal of Analysis Laboratory

基　　金：陕西省教育厅科学研究专项项目(14JK1826);延安大学化学与化工学院专项(YDHG2014-Z03)资助

摘　　要：阿米卡星诱导金纳米粒子的快速团聚,使其在520 nm处吸收值降低,同时在640 nm附近产生新吸收,且两处吸光度比值（A640/A520）的变化与阿米卡星的浓度呈正比,据此建立了测定阿米卡星的新方法。对p H、反应时间以及纳米金浓度等实验条件进行了优化,在优化实验条件下,测定阿米卡星的线性范围为2.0×10-7~5.0×10-6mol/L,检出限为0.6×10-8mol/L,相对标准偏差（RSD）为1.9%（n=11）。方法用于硫酸阿米卡星注射液的测定,回收率在100.0%~105.0%。A simple,rapid and highly sensitive spectrophotometric method was developed for determination of trace amounts of amikacin. The presence of amikacin caused the aggregating of gold nanoparticles（ Au NPs） and thus changed the ratio value of A640/ A520,which was used as a basis for the quantitative determination of amikacin. The effect of various experimental factors such as p H,concentrations of Au NPs and reaction time,were studied to optimize the reaction conditions. Under optimum conditions,the calibration graphs were linear within the range of 2. 0 × 10- 7~ 5. 0 × 10- 6mol/L and the detection limit was 6. 0 × 10- 9mol/L with relative standard deviation（ RSD） of 1. 9%（ n = 11）. The method was successfully applied to the determination of amikacin in amikacin injections,and the recovery was between 100. 0% ~ 105. 0%.

关 键 词：比色法 纳米金 阿米卡星 

分 类 号：R917[医药卫生—药物分析学]