高选择性半胱氨酸探针的设计、合成及光谱性质研究  

Design,synthesis and spectra studies of highly selective fluorescent probe for cysteine

作　　者：杨志广[1] 石晓明[1] 彭鹏[1] 李帅兵[1] 田明刚[2] 

机构地区：[1]周口师范学院化学化工学院,周口466001 [2]山东大学晶体材料国家重点实验室,济南250100

出　　处：《分析试验室》2015年第8期950-953,共4页Chinese Journal of Analysis Laboratory

基　　金：周口师范学院博士科研启动经费资助项目(zksybscx201202);河南省高等学校重点科研项目(15A430055)资助

摘　　要：合成了一种新型的裸眼识别半胱氨酸(Cys)的荧光探针,可以专一性地识别半胱氨酸而不受同型半胱氨酸(Hcy)、二硫苏糖醇(DTT)、谷胱甘肽(GSH)和其它氨基酸的影响。研究结果表明:探针与Cys作用后,溶液颜色由无色变为黄色,紫外吸收光谱红移约32 nm,同时荧光强度淬灭,荧光光谱红移约75 nm,可实现裸眼检测。A novel naked-eye and fluorescent probe for cysteine detection has been designed and synthesized successfully,which showed an excellent selectivity for cysteine over homocysteine, dithiothreitol（ DTT）,glutathione（ GSH） and other amino acids. The free probe was colorless and fluorescent,Upon addition of Cys,however it has become yellow with a ~ 32 nm red-shift in absorption spectra and its fluorescent intensity has been quenched with a 75 nm red-shift in fluorescence spectra,which can realize the naked eye detection for cysteine.

关 键 词：半胱氨酸 荧光探针 裸眼识别 选择性 

分 类 号：O621.3[理学—有机化学] O657.3[理学—化学]