基质辅助激光解析电离飞行时间质谱技术在快速鉴定真菌中的临床应用研究  

Analysis of the clinical application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry technology in the rapid identification of pathogenic fungi

作　　者：叶丽艳[1] 郭玲[1] 马艳宁[1] 张樱[1] 赵强[1] 李丰田[1] 王蕊[1] 杨继勇[1] 王成彬[1] 罗燕萍[1] 

机构地区：[1]解放军总医院微生物科,北京100853

出　　处：《中华医院感染学杂志》2015年第15期3361-3363,共3页Chinese Journal of Nosocomiology

基　　金：国家重大科学仪器设备开发专项(2012YQ18011708)

摘　　要：目的总结基质辅助激光解析电离飞行时间质谱技术(MALDI-TOF MS)应用于临床真菌鉴定的特点,为评估其在真菌快速鉴定方面的重要性及临床应用价值。方法采用VITEK MS鉴定仪对2013年1月-2014年9月临床检出真菌进行鉴定,获取真菌全细胞蛋白(主要是核糖体蛋白)质谱,通过与数据库中已知菌质谱进行比较、分析,获得鉴定结果并对数据进行统计分析。结果共鉴定出真菌1 254株,质谱技术鉴定真菌共1 198株,占95.53%,未鉴定出真菌占4.47%;应用质谱技术鉴定的真菌中以假丝酵母菌属为主,共1 125株占93.91%。结论 MALDI-TOF MS技术鉴定真菌种类多,操作流程简单、快速,而且还具有很好的可重复性和准确性,是传统真菌鉴定方法的有力补充,为真菌鉴定提供了新的选择。OBJECTIVE To summarize the characteristics of the matrix assisted laser desorption ionization time‐of‐flight mass spectrometry technology （MALDI‐TOF MS） in the quick identification of clinical pathogenic fungi so as to evaluate the clinical significance and application value of MALDI‐TOF MS in the rapid identification of fungi . METHODS The clinical isolates of fungi from Jan .2013 to Sep .2014 were identified by VITEK MS .The whole cell protein （mainly ribosomal protein） mass spectrometry of the fungi were obtained .They were compared with the fungal mass spectrometry in the database and the results were statistically analyzed .RESULTS Totally 1198 strains of fungi in the identified 1254 strains were identified by MS ,accounting for 95 .53% and others were not i‐dentified by it . Candidias dominated the majority of the fungi identified by MS with 1125 strains , holding 93 .91% .CONCLUSION MALDI‐TOF MS was a good method to identify fungi simply and quickly .The identifi‐cation spectrum of MALDI‐TOF MS was extensive .MALDI‐TOF MS also had good repeatability and accuracy .It is a powerful complement to traditional methods for identification of fungi and provides a new choice for fungal i‐dentification .

关 键 词：基质辅助激光解析电离飞行时间质谱 真菌 快速鉴定 

分 类 号：R446[医药卫生—诊断学]