炎症性肠病患者肠道菌群变化与发病关系研究  

Study on relationship of intestinal flora changes and pathogenesis in patients with inflammatory bowel disease

作　　者：宋军民[1] 杨超[1] 常远[1] 夏坤锟[1] 王朝杰[2] 

机构地区：[1]郑州大学第一附属医院肛肠外科,河南郑州450052 [2]河南省人民医院肿瘤科,河南郑州450003

出　　处：《中华医院感染学杂志》2015年第15期3425-3427,共3页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(U1204818)

摘　　要：目的探讨炎症性肠病患者肠道菌群结构变化与相关炎性指标的关系,为临床治疗提供参考依据。方法选取2013年1月-2014年3月于医院就诊的121例溃疡性结肠炎患者和34例克罗恩病患者为研究对象,57名正常志愿者为对照组,采集受试者的新鲜粪便进行菌群分析,比较菌群变化与炎性指标的相关性,采用SPSS18.0软件进行统计分析。结果与对照组相比,溃疡性结肠炎患者肠内酵母菌属(2.29±0.41)CFU/g、双歧杆菌属(5.20±0.53)CFU/g、乳杆菌属(5.62±0.12)CFU/g、肠球菌属(6.58±0.23)CFU/g、拟杆菌属(5.64±0.28)CFU/g、消化球菌属(6.35±0.25)CFU/g、小梭菌(3.61±0.30)CFU/g的数量明显增加,而真杆菌属(1.62±0.24)CFU/g的数量下降,差异均有统计学意义(P<0.05);培养结果阳性检出率与菌群数量变化结果基本相附;而菌群与炎性指标有相关性。结论炎症性肠病患者的肠道菌群失衡,其发病与真杆菌属数量的减少有关。OBJECTIVE To discuss the correlation between the changes of intestinal flora and inflammatory indica‐tors in patients with inflammatory bowel disease ,so as to provide basis for clinical treatment .METHODS Totally 121 cases of ulcerative colitis and 34 cases of Crohn′s disease in our hospital from Jan .2013 to Mar .2014 were en‐rolled as subjects in this study with 57 healthy people as the control group .Fresh feces of subjects were collected for analysis to assess the correlation between the changes of flora and inflammatory indicators . The software SPSS18 .0 was used for statistical analysis .RESULTS Compared with the control group ,Saccharomyces （2 .29 ± 0 .41） CFU/g ,Bi f idobacterium （5 .20 ± 0 .53） CFU/g ,L actobacillus （5 .62 ± 0 .12） CFU/g ,Enterococcus （6 .58 ± 0 .23） CFU/g ,Bacteriodes （5 .64 ± 0 .28） CFU/g ,Clostridium （3 .61 ± 0 .30） CFU/g ,and Peptococcus （6 .35 ± 0 .25） CFU/g in ulcerative colitis patients all significantly increased ,while Eubacterium （1 .62 ± 0 .24） CFU/g reduced significancely （P〈0 .05） .The results of quantitative analysis of flora were consistent with the positive rates of bacterial culture .The inflammatory markers did not show a significant correlation with the changes in flo‐ra count .CONCLUSION The progression of intestinal flora imbalance in patients with inflammatory bowel disease is associated with the decrease of number of Eubacterium .

关 键 词：炎症性肠病 肠道菌群 炎性指标 

分 类 号：R378[医药卫生—病原生物学]