补肾疏肝汤对大鼠抗疲劳的作用机制研究  

Investigation of anti-fatigue effect of Bushen-shugan decoction in rats and its mechanism

作　　者：牛占忠[1] 张喜平[2] 周红军[1] 王慧[1] 

机构地区：[1]沧州医学高等专科学校针推教研室,河北沧州061000 [2]沧州医学高等专科学校基础护理教研室,河北沧州061000

出　　处：《河北中医》2015年第9期1363-1365,共3页Hebei Journal of Traditional Chinese Medicine

基　　金：河北省沧州市科技局2011年沧州市科学技术研究与发展指导计划(编号:1123079ZD)

摘　　要：目的观察补肾疏肝汤对疲劳大鼠血红蛋白、肝糖原及肌糖原含量的影响,探讨其抗疲劳的作用机制。方法将50只雄性健康SD大鼠随机分为5组,即空白对照组、模型对照组及补肾疏肝汤低、中、高剂量组,每组各10只。除空白对照组外,其余4组大鼠采用力竭游泳加剥夺睡眠法制备疲劳大鼠模型,同时予相应药液40mL/(kg·d)灌胃。14 d后取各组大鼠主动脉血,检测大鼠血红蛋白、肝糖原和肌糖原水平,进行比较。结果与模型对照组比较,补肾疏肝汤低、中、高剂量组血红蛋白、肝糖原及肌糖水平均升高(P<0.05,P<0.01),其中补肾疏肝汤中剂量组血红蛋白水平高于补肾疏肝汤低、高剂量组(P<0.05),补肾疏肝汤高、中剂量组肝糖原、肌糖原水平高于补肾疏肝汤低剂量组(P<0.05)。结论补肾疏肝汤能通过提高疲劳大鼠血红蛋白、肝糖原和肌糖原水平而发挥抗疲劳作用。Objective To investigate the effect of Bushen - shugan decoction on the contents of hemoglobin, hepatic glycogen and muscle glycogen in fatigued rats, and its mechanism of anti - fatigue. Methods 50 healthy male SD rats were randomly divided into five groups that blank control group, model control group, Bushen - shugan decoction low dose,middle dose and high dose group, 10 rats in each group. Except the blank control group, the fa- tigued rat models were induced by exhaustive swimming and sleep deprivation in other four groups, and received corre- sponding medicine 40 mL/（ kg · d）by gavage. The aortic blood was collected after 14 days in all groups, and the con- tents of hemoglobin, hepatic glycogen and muscle glycogen were detected and compared. Results As compared with model control group ,the contents of hemoglobin, hepatic glycogen and muscle glycogen in Bushen- shugan decoction low dose, middle dose and high dose groups were increased （ P 〈 0. 05 ）, and the hemoglobin content in Bushen - shu- gan decoction middle dose group was higher than that in Bushen - shugan decoction low dose and high dose group （P 〈 0.05 ）, the contents of hepatic glycogen and muscle glycogen in Bushen - shugan decoction middle dose and high dose group were higher than those in Bushen - shugan decoction low dose group （P 〈 0.05）. Conclusion Bushen - shugan decoction can increase the contents of hemoglobin, hepatic glycogen and muscle glycogen in fatigued rats, thus play the anti -fatigue role.

关 键 词：疲劳综合征 慢性 疾病模型 动物 大鼠 补肾 疏肝 血红蛋白 肝糖原 

分 类 号：R872.7[医药卫生—运动医学] R285.5[医药卫生—临床医学]