防己水煎液及其不同拆分部位对脂多糖诱导下RAW264.7细胞炎症因子的影响  

Effect of Inflammatory Cytokines in the LPS-induced RAW264.7 Cells by the Decoction and Its Split Components from Stephania tetrandra S. Moore

作　　者：郭辰[1] 王蒙[1] 李静[1] 魏晴[1] 王秋红[1] 杨炳友[1] 匡海学[1] 

机构地区：[1]黑龙江中医药大学北药基础与应用研究省部共建教育部重点实验室黑龙江省中药及天然药物药效物质基础研究重点实验室,黑龙江哈尔滨150040

出　　处：《中医药学报》2015年第4期33-36,共4页Acta Chinese Medicine and Pharmacology

基　　金：国家重点基础研究发展计划(973计划)(2013CB531801)

摘　　要：目的:观察防己水煎液及其不同拆分部位对脂多糖(LPS)诱导下RAW264.7细胞炎症因子的影响。方法:采用噻唑蓝比色法(MTT法)测定防己水煎液及其不同拆分部位对RAW264.7细胞的毒性作用,格里斯氏试剂(Griess法)测定细胞炎症反应产生的NO含量,酶联免疫吸附法(ELISA)检测细胞上清液中肿瘤坏死因子-α(TNF-α),白介素-6(IL-6)的含量。结果:防己水煎液及其生物碱拆分组分可抑制LPS诱导的RAW264.7细胞释放细胞炎症因子NO(P<0.01),TNF-α(P<0.01),IL-6(P<0.01),其他组分无明显效果。结论:防己水煎液及其不同拆分部位在浓度小于312.50mg/L的范围内对RAW264.7细胞无显著毒性。防己水煎液及其生物碱拆分组分可能通过抑制细胞因子NO,TNF-α,IL-6的释放发挥其抗炎作用。Objective：To observe the effect of inflammatory factors from decoction of Stephania tetrandra S.Moore and its different split components that lipopolysaccharide （LPS）was induced by RAW264.7 cells.Methods：Use MTT method to determine the cytotoxicity of different parts from the decoction and its split components of RAW264.7 cells.Use Gries＆#39;reagent （ Griess method） to determine the NO content of the inflammatory reaction cell and use the method of enzyme-linked immunosorbent （ELISA） to detect tumor necrosis factor alpha （TNF-α） and interleukin 6 （IL-6） content in the supernatant fluid.Results： The decoction and its alkaloids components could inhibit LPS induced by RAW264.7 cells releasing inflammatory factor NO （ P〈0.01） , the TNF-α（ P〈0.01） , IL-6 （ P〈0.01） and other components had no obvious effects.Conclusion：The concentrations of decoction and its different parts are less than 312.50mg/L for RAW264.7 cells and have no significant toxicity.The decoction and its alkaloids split components may inhibit the re-lease of cytokines NO, TNF-α, IL-6 and exert its anti-inflammatory effects.

关 键 词：防己 炎症 小鼠巨噬细胞 酶联免疫吸附试验 细胞因子 

分 类 号：R285.5[医药卫生—中药学]