归芪多糖对衰老细胞周期及p53、p16蛋白的影响  

Effect of Guiqi Polysaccharide on Cell Cycle and Protein Expression of p53 and p16 in Senescence Cells

作　　者：蒲秀瑛[1] 于双[1] 樊文博[1] 任菁[1] 刘璐[1] 马小龙[1] 张伟杰[1] 

机构地区：[1]兰州理工大学生命科学与工程学院甘肃省中藏药筛选评价及深加工重点实验室,甘肃兰州730050

出　　处：《中医药学报》2015年第4期36-39,共4页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(81260070);兰州市科技局项目(2011-1-71)

摘　　要：目的:研究归芪多糖对衰老的WI-38细胞的细胞周期及p53、p16蛋白表达的影响。方法:采用MTT法,研究不同浓度归芪多糖对WI-38细胞活力的影响,筛选出归芪多糖最佳作用浓度;采用流式细胞术分析细胞周期;Western blot法分析归芪多糖对p53、p16蛋白表达的影响。结果:0.016/mg·m L归芪多糖可明显促进细胞增殖;与衰老组细胞相比,归芪多糖可明显促进衰老细胞进入分裂期(P<0.01),并可显著降低衰老模型组细胞p53和p16蛋白的表达。结论:归芪多糖可延缓WI-38细胞的衰老,其作用机制与调控细胞周期,抑制p53-p21和p16-pRb信号通路有关。Objective：To investigate the effect of Guiqi polysaccharide （ GQP） on the cell cycle and the protein expres-sion of p53 and p16 in senescence cells.Methods：MTT assay was used to observe the effect of different concentrations of GQP on the proliferation of WI-38 cells and screen the best concentration of GQP to promote the growth of WI-38 cells.Cell cycle was analyzed by flow cytometry, and Western blot analysis was performed to evaluate the protein ex-pression of p53 and p16 in senescence cells.Results：0.016mg/mL GQP was the best concentration of promoting cell proliferation.Compared with H2 O2 model group, GQP could promote the senescence cells from G1-phase to S-phase and reduce the protein expression of p53 and p16, and the difference was significant （ P〈0.01） .Conclusion：GQP has an protective effect on senescence cells.The anti-aging effect of GQP may be involved in regulating the cell cycle and weakening two signal pathways （ p53-p21 and p16-pRb） .

关 键 词：归芪多糖 WI-38细胞 细胞周期 

分 类 号：R285.5[医药卫生—中药学]