姜炭炮制前后对虚寒性出血症大鼠尿液代谢组学分析  

Urine Metabonomics Analysis of Dried and Charred Zingiberis Rhizoma Recens on Rats with Deficiencycold Hemorrhagic Disease

作　　者：莫毛燕 朱琼花[1] 薛兴阳[2] 邓仙梅 周苏娟[1] 孟江[1] 王淑美[1] 

机构地区：[1]广东药学院,广州510006 [2]广州医科大学附属肿瘤医院,广州510000

出　　处：《中国实验方剂学杂志》2015年第16期1-4,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81473352)

摘　　要：目的:运用代谢组学研究姜炭炮制前后对虚寒性出血症大鼠尿液的代谢表型变化,探讨姜炭炮制前后药效及作用机制的变化。方法:利用核磁共振氢谱(1H-NMR)技术建立大鼠尿液的代谢指纹谱,应用主成分分析(PCA)和偏最小二乘判别分析(PLS-DA)研究空白组与模型组、姜炭组、干姜组相互间的代谢物谱差异,通过变量重要性投影(VIP)在尿液中发现潜在的生物标志物,通过矫正率筛选姜炭温经止血的药效生物标志物。结果:姜炭可使虚寒性出血证大鼠发生紊乱的内源性生物标志物回归正常水平,而干姜作用较弱。筛选出与虚寒性出血症相关的7种生物标志物,其中乙酰乙酸、丙酮酸、氮氧三甲胺、牛磺酸、色氨酸为姜炭温经止血的药效生物标志物。结论:代谢组学研究能较全面地反应生物体的生理及代谢状态,为中医"证"的本质研究及中药炮制前后药效评价提供新方法。Objective： To investigate urine metabolic profile of rats with deficiency-cold hemorrhagic disease,and discuss intervention effects of dried and charred Zingiberis Rhizoma Recens. Method： Proton nuclear magnetic resonance（^1H-NMR） technique was used to analyze metabolome in urine. Partial least squares discriminant analysis（ PLS-DA） was processed to analyze metabonome difference among four groups. Seven potential biomarkers were selected by variable importance in the projection（ VIP）. Principal component analysis（ PCA） was employed to process data from the charred Zingiberis Rhizoma Recens treatment group,hemostatic effects of charred Zingiberis Rhizoma Recens were investigated through the content of potential biomarkers. Result：Charred Zingiberis Rhizoma Recens could make disorder endogenous biomarkers of the model rats to normal levels,while dried Zingiberis Rhizoma Recens had weaker efficacy. Acetoacetic acid, pyruvic acid, nitrogen oxygen trimethylamine,taurine and tryptophan were efficacy of hemostasis biomarkers in charred Zingiberis Rhizoma Recens. Conclusion： Metabolomics can reflect the whole status of metabolism in biosystems,it can be treated as a potential powerful approach in pharmacological studies for different processed products and investigation of essence of syndrome in traditional Chinese medicine.

关 键 词：姜炭 虚寒性出血证 代谢组学 核磁共振 尿液 大黄 

分 类 号：R945[医药卫生—微生物与生化药学] R283.3[医药卫生—药剂学]