正交试验优选巴戟天饮片的软化和切制工艺  

Optimization for Softening and Cutting Processes of Morindae Officinalis Radix by Orthogonal Design

作　　者：黄玉秋[1] 贾天柱[1] 史辑[1] 

机构地区：[1]辽宁中医药大学药学院,国家中医药管理局炮制原理解析重点研究室,辽宁省中药炮制工程技术研究中心,辽宁大连116600

出　　处：《中国实验方剂学杂志》2015年第16期5-9,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81001635,81473350);国家发改委行业专项(201107007)

摘　　要：目的：优化巴戟天的软化和切制工艺,为该饮片的规范生产提供技术参数。方法：以甲基异茜草素-1-甲醚、水晶兰苷和耐斯糖的综合评分为指标,在单因素试验基础上,通过正交试验考察加水量、闷润时间、闷润温度、切段长度对巴戟天软化切制工艺的影响。采用HPLC测定甲基异茜草素-1-甲醚的含量,流动相乙腈-0.2%磷酸水溶液梯度洗脱,检测波长277 nm;水晶兰苷含量测定的流动相甲醇-0.1%磷酸水溶液梯度洗脱,检测波长235 nm。采用高效液相色谱电雾式检测器法（HPLC-CAD）测定耐斯糖含量,流动相甲醇-水（3∶97）。结果：最佳软化切制工艺为加1.0倍量水于50℃下闷润9 h,切段长度10～15 mm。甲基异茜草素-1-甲醚、水晶兰苷、耐斯糖及水浸出物平均质量分数分别为0.045%,5.074%,63.202%,74.651%。结论：优选的巴戟天软化切制工艺合理可行,可作为该饮片的规范生产工艺。Objective： To optimize softening cutting processes of Morindae Officinalis Radix,in order to provide technical parameters for standard production of the pieces. Method： On the basis of single-factor test,with comprehensive score of rubiadin-1-methyl ether,monotropein and nystose as indexes,orthogonal design was adopted to detect effects of water volume,moistening time,moistening temperature and cutting length on softening and cutting processes. HPLC was used to determine the content of rubiadin-1-methyl ether,which was eluted with mobile phase of acetonitrile-0. 2% aqueous phosphoric acid in gradient mode,detection wavelength was 277 nm.The content of monotropein was eluted with mobile phase of acetonitrile-0. 1% aqueous phosphoric acid in gradient mode,detection wavelength was 235 nm. HPLC-CAD was used to determine the content of nystose with mobile phase of methanol-water（ 3∶ 97）. Result： Optimal softening and cutting processes were as follows： water volume of 1. 0 times,moistening temperature at 50 ℃,moistening time of 9 h and cutting length of 10-15 mm. Average fractions of rubiadin-1-methyl ether,monotropein,nystose and water extract were 0. 045%,5. 074%,63. 202%and 74. 651%, respectively. Conclusion： These optimized softening and cutting processes for Morindae Officinalis Radix are rational and feasible,it can be used as technical parameters for standard production of the pieces.

关 键 词：巴戟天 软化 切制 甲基异茜草素-1-甲醚 水晶兰苷 耐斯糖 

分 类 号：R283.3[医药卫生—中药学] R284.1[医药卫生—中医学]