四物汤对急性血瘀模型大鼠活血祛瘀有效部位筛选  

Screening of Active Fractions of Siwu Tang on Acute Blood Stasis Model Rat

作　　者：贾梅[1] 郑传柱[1] 张丽[1] 丁安伟[1] 

机构地区：[1]南京中医药大学江苏省方剂高技术研究重点实验室,江苏省中药资源产业化过程协同创新中心,南京210023

出　　处：《中国实验方剂学杂志》2015年第16期119-123,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省高校自然科学研究项目(12KJA360002);江苏高校优势学科建设工程项目(ysxk-2014)

摘　　要：目的:比较四物汤不同溶剂提取部位对急性血瘀模型大鼠血液流变学、凝血功能和血小板系统的影响,筛选四物汤活血祛瘀有效部位。方法:四物汤经水煎煮后,采用系统溶剂提取法分别得到4个溶剂提取部位。雌性SD大鼠80只分为8组,每组10只,分别为正常组,模型组,复方丹参滴丸组(0.15 g·kg-1),四物汤组(7 g·kg-1),石油醚提取液组(7 g·kg-1),乙酸乙酯提取液组(7 g·kg-1),正丁醇提取液组(7 g·kg-1),水提取液组(7 g·kg-1)。除正常组外,其余各组采用ih盐酸肾上腺素加冰水浴复制大鼠急性血瘀模型,比较四物汤不同提取部位给药后大鼠血液流变学,凝血酶原时间(PT),凝血酶时间(TT),活化部分凝血活酶时间(APTT),血浆纤维蛋白原(FIB),血小板聚集率,血浆中血栓素B2(TXB2)和6-酮-前列腺素F1a(6-keto-PGFla)含量的差异。结果:与正常组比较,模型组各切变率全血黏度、血浆黏度明显升高,TT,PT,APTT明显缩短,FIB含量明显升高,血小板聚集率明显升高,血浆TXB2含量明显升高,6-keto-PGFla的含量明显降低(P<0.01);与模型组比较,四物汤乙酸乙酯组明显降低大鼠各切变率全血黏度、血浆黏度,明显延长TT,PT,APTT,明显降低FIB含量、血小板聚集率、血浆TXB2的含量,明显升高6-keto-PGFla的含量(P<0.01);石油醚组可明显降低高切变率下全血黏度(P<0.05),降低TXB2含量、升高6-keto-PGFla的含量(P<0.01);正丁醇组、水提组可明显降低TXB2含量、升高6-keto-PGFla含量(P<0.01)。结论:乙酸乙酯部位是四物汤活血祛瘀的最有效部位。Objective： To screen active fractions of Siwu Tang for activating blood and removing stasis by comparing their effects on the blood rheology,function of blood coagulation and platelet system of the acute model rats. Method： After Siwu Tang was boiled with water,the system solvent extraction method was adopted to extract four fractions respectively. Totally 80 female SD rats were divided into eight groups： the control group,the model group,the Compound Danshen dropping pill group（ 0. 15 g·kg^-1）,the Siwu Tang group（ 7 g·kg^-1）,the Petroleum ether extract group（ 7 g·kg^-1）,the Ethyl acetate extract group（ 7 g·kg^-1）,the N-butyl alcohol extract group（ 7 g·kg^-1） and the water extract group（ 7 g·kg^-1）. Except for the normal group,the ice water bath and subcutaneous injection with adrenaline hydrochloride were applied in all of the rest groups to establish the acute blood stasis rat model,in order to compare different extracted fractions in the hemorheology of rats after the administration,prothrombin time（ PT）,thrombin time（ TT）,activate parts thrombin time（ APTT）,fibrinogen（ FIB）,platelet aggregation rate,plasma thrombox-ane B2（ TXB2） and 6-keto-prostaglandin Fla（ 6-ketoPGFla）. Result： Compared with the normal group,the model group showed significant decreases in the shear rate of whole blood viscosity,plasma viscosity,TT,PT,APTT and 6-keto-PGFlaand significant increases in FIB,platelet aggregation rate,plasma TXB2. Compared with the normal group,ethyl acetate fractions of Siwu Tang can significantly reduce the shear rate of whole blood viscosity,plasma viscosity,significantly extend TT,PT,APTT,significantly reduce FIB,platelet aggregation rate,plasma TXB2,elevate 6-keto-PGFla; the Petroleum ether group can notably decrease the whole blood viscosity at a high shear rate（ P〈0. 05）,reduce TXB2 content,increase 6-keto-PGFla content（ P〈0. 01）; N-butyl alcohol fractions and water extracts can obviously reduce TXB2 and increase 6-keto-PGFla（ P〈

关 键 词：四物汤 活血祛瘀 部位筛选 

分 类 号：R285.5[医药卫生—中药学]