青娥方提取物对D-半乳糖致衰老小鼠的延缓衰老作用及机制  

Anti-aging Effect and Mechanism of Qing'e Prescription Extract on Aging Mice Induced by D-galactose

作　　者：钟琳 王晓舜[2] 王婷婷[1] 吴辉[1] 吴晓俊[1] 尉小慧[1] 王峥涛[1] 

机构地区：[1]上海市复方中药重点实验室暨上海中医药大学中药研究所,上海201203 [2]青岛市市立医院药学部,山东青岛266000

出　　处：《中国实验方剂学杂志》2015年第16期134-138,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：上海市中医药事业发展三年行动计划项目(ZYSNXD-GH-FFYJ)

摘　　要：目的:探讨青娥方提取物对D-半乳糖致衰老模型小鼠的延缓衰老作用。方法:将小鼠随机分为空白组、衰老模型组、阳性药组、青娥方提取物低、中、高剂量组。除空白组颈背部皮下注射生理盐水外,其余各组均颈背部皮下注射D-半乳糖溶液,空白组和模型组均用0.5%羧甲基纤维素钠水溶液灌胃;阳性药组维生素E(0.1 g·kg-1)及青娥方提取物低、中、高剂量组(0.4,0.8,1.6 g·kg-1)剂量的青娥方提取物的羧甲基纤维素钠水溶液灌胃6周后进行转棒仪疲劳实验;同时测定肝脏中谷胱甘肽(GSH),丙二醛(MDA),总抗氧化能力(T-AOC),一氧化氮(NO),一氧化氮合酶(NOS)等抗氧化生化指标;血清中尿素氮(BUN),乳酸脱氢酶(LDH)等抗疲劳生化指标;并采用实时定量PCR检测海马抗氧化基因锰超氧化物歧化酶(Mn SOD),过氧化物酶-3(Prdx-3)mRNA的表达。结果:转棒疲劳仪行为学实验,与空白组比较,模型组小鼠在转棒停留时间明显缩短(P<0.01)。青娥方提取物各剂量组与模型组相比,均可以显著延长小鼠在转棒仪上的停留时间(P<0.05,P<0.01)。生化指标测定模型组血清与空白组相比,BUN,MDA,NO,NOS的含量均升高(P<0.05),而LDH含量降低(P<0.05);各给药组均能明显改善上述指标(P<0.05,P<0.01)。肝组织生化指标,与空白组GSH含量及T-AOC比较模型组均显著降低(P<0.01),MDA,NO,NOS含量均显著升高(P<0.05,P<0.01),而给药组较模型组相比,各指标均显著改善(P<0.05,P<0.01)。实时定量PCR结果显示,模型组海马Mn SOD mRNA表达水平较空白组降低(P<0.05),给药组与模型组相比,海马Mn SOD,Prdx-3 mRNA的表达显著提高(P<0.05,P<0.01)。结论:青娥方提取物能显著提高机体抗氧化能力,发挥延缓衰老和抗疲劳作用。Objective： To study the anti-aging effect and mechanism of Qing＇e prescription（ QEP）extract on aging mice induced by D-galactose. Method： Seventy-two KM male mice were equally divided into six groups randomly： control group,model group,vitamin E group,and low-（ 0. 4 g·kg^-1）,middle-（ 0. 8 g·kg^-1）,and high-（ 1. 6 g·kg^-1） doses of QEP extract groups. To establish the subacute aging model,all the groups except control were subcutaneously injected with D-galactose at the back of cervical region daily. The control group and model group were intragastrically administered with 0. 5% sodium carboxymethyl cellulose solution. The vitamin E group,low-,middle-,and high-doses of QEP extract groups received consecutive Vitamin E and low-,middle-,and high-doses of QEP extract prepared in sodium carboxymethyl cellulose solution,respectively,for six weeks. After the treatment,the anti-aging and anti-fatigue effects were evaluated by conducting the rotarod test,and measuring the contents of glutathione（ GSH）,malondialdehyde（ MDA）,total antioxidant capacity（ TAOC）, nitric oxide（ NO） and nitric oxide synthase（ NOS） in liver, urea nitrogen（ BUN） and lactate dehydrogenase（ LDH） in serum together with other biochemical indexes. Moreover,the expression of Manganese superoxide dismutase（ Mn SOD） and Peroxidase-3（ Prdx-3） mRNA in hippocampus was analyzed by q PCR to evaluate the antioxidant level. Result： Rotarod test showed that model group mice was easier to fall from the rotarod（ P〈0. 01） in comparison with the control group. Compared with the model group,all the doses of QEP extract could significantly prolong the residence time of mice on the rod（ P〈0. 05,P〈0. 01）. Biochemical tests showed that serum levels of BUN,MDA,NO,NOS in model group were increased remarkably when compared with that in the control group（ P〈0. 05）. By contrast,serumLDH level was reduced（ P〈0. 05）. After treated with QEP extract,all the aforementioned indexes were improve

关 键 词：青娥方 D-半乳糖 抗氧化 延缓衰老 

分 类 号：R285.5[医药卫生—中药学]