纸基-表面增强拉曼光谱法检测染色掺伪的红花药材  

Paper-based SERS wiper for detection of the dyed safflower

作　　者：李丹[1,2] 吕狄亚[1] 陈辉[1,2] 陆峰[1,2] 陈强[2] 褚克丹[2] 

机构地区：[1]第二军医大学,上海200433 [2]福建中医药大学,福州350108

出　　处：《药物分析杂志》2015年第8期1466-1470,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立一种纸基-表面增强拉曼光谱方法对染色掺伪的红花进行检测。方法:用浸泡法制备的银胶纸作为拭子擦拭经乙醇溶液润湿的红花,对拭子的擦拭区进行SERS检测。先后对拭子的浸泡时间、干燥条件和乙醇溶液的浓度等因素进行优化。结果:成功检测经甲基红、碱性品红、金胺O和对氨基偶氮苯4种常见染料染色的红花药材。结论:纸基-表面增强拉曼光谱法可实现对染色红花的快速、简单、灵敏和无损的检测,并满足现场快检的需求。Objective ：To establish a paper-based surface-enhanced Raman scattering（SERS） method for the detection of dyed safflower. Methods：The Ag NPs-wipers were synthesized by soaking method. The dried wipers were used to wipe the dyed safflower wetted by ethanol, and then the wiper zones were immediately detected by a portable Raman spectrometer. Factors including the dipping time and drying type of wiper, concentration of ethanol were in- vestigated and optimized. Results：The dyed safflowers, stained by methyl red, basic fuchsin, auramine O and p -ami- noazobenzene, were successfully detected using the established method. Conclusion： The combination of Ag NPs- wiper and SERS technology enables a rapid, simple, sensitive and non-destructive detection, which fits the requirements of large field tests.

关 键 词：银胶拭子 拉曼光谱 红花 染料 染色掺伪 甲基红 碱性品红 金胺O 对氨基偶氮苯 

分 类 号：R917[医药卫生—药物分析学]