固相萃取-加压毛细管电色谱法测定水体中8种农药残留  

Determination of Eight Pesticide Residues in Water by Solid Phase Extraction-Pressurized Capillary Electrochromatography

作　　者：山梅 李静[1,2] 茹鑫[1,2] 郑署 王晓曦[1] 闫超[1] 

机构地区：[1]上海交通大学药学院,上海200240 [2]上海通微分析技术有限公司,上海201203

出　　处：《分析科学学报》2015年第4期445-449,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21175092;21105064);国家重大科学仪器设备开发项目(No.2011YQ150072;2011YQ15007204;2011YQ15007210);上海市自然科学基金(No.12ZR1313600);苏州市科技支撑计划(No.SG201214);国家科技型中小企业创新基金(No.12C26213202212)

摘　　要：采用固相萃取-反相加压毛细管电色谱-紫外检测技术，建立了水体中乐果、敌敌畏、克百威、甲萘威、莠去津、甲基对硫磷、马拉硫磷、百菌清8种农药残留的同时检测方法。在最佳条件下，8种目标农药的线性范围分别为：3．4～100μg／mL、8．1～120μg／mL、1．2～50μg／mL、0．2～50μg／mL、0．1～50μg／mL、3．7～100μg／mL/。11．2～150μg／mL,2．1～100μg／mL，相关系数为0．9961～0．9997；检出限（S／N=3）在0．03～3．7μg／mL之间，加标回收率在71．09／6～114．1％范围，相对标准偏差（RSD）为1．1％～9．8％。该方法简单、可靠、适用于水中多种农药残留的同时分析测定。By combining solid phase extraction,pressurized capillary electrochromatography（pCEC）equipped with ultra-violet detector was used to determine eight pesticide residues including dimethoate,dichiorvos,carbofuran,carbaryl,atrazine,methyl parathion and malathion and chlorothalonil in water via a reversed-phase C18 column.Under the optimal separation condition,the linear ranges of the eight pesticides were 3.4-100μg/mL,8.1-120μg/mL,1.2-50μg/mL,0.2-50μg/mL,0.1-50μg/mL,3.7-100μg/mL,11.2-150μg/mL and 2.1-100μg/mL,repectively.Correlation coefficients were between0.9961 and 0.9997,and limits of detection were between 0.03 and 3.7μg/mL.Average recoveries between71.0% and 114.1%and relative standard deviations between 1.1%and 9.8% were obtained.It is demonstrated that the pCEC-UV can be used for the determination of pesticides in water sample.

关 键 词：加压毛细管电色谱 紫外检测 固相萃取 农药残留 毛细管电色谱柱 

分 类 号：O657.8[理学—分析化学]