N,N-二(2-羧基苯基)-2,6-吡啶二甲酰胺与牛血清白蛋白作用的荧光光谱研究  

Study on the Interaction of N,N-Bis(2-carboxylphenyl)-2,6-Pyridinedicarboxamide with Bovine Serum Albumin by Fluorescence Spectrometry

作　　者：王彩荣[1] 王璟琳[1] 李敏[1] 王丹[1] 

机构地区：[1]长治学院化学系,山西长治046011

出　　处：《分析科学学报》2015年第4期529-532,共4页Journal of Analytical Science

基　　金：山西省高校科技开发课题(No.2013159);长治学院科研课题(201416);山西省大学生创新项目(No.2014435)

摘　　要：在pH=7.4的Tris-HCl缓冲溶液中,采用荧光光谱法研究了N,N-二(2-羧基苯基)-2,6-吡啶二甲酰胺(BCPD)与牛血清白蛋白(BSA)的相互作用。结果表明,BCPD可以动态猝灭BSA的内源荧光,结合位点数约为1;测得291K、296K、301K和306K四个温度下两者的平均结合常数为3.82×106 L·mol-1;热力学参数表明两者之间结合的作用力为疏水作用。The interaction between N,N-bis（2-carboxylphenyl）-2,6-pyridinedicarboxamide（BCPD）and bovine serum albumin（BSA）was investigated by fluorescence spectrometry in pH =7.4 Tris-HCl buffer.The results showed that the fluorescence of BSA was quenched by BCPD and its mechanism was a dynamic quenching.And,the binding constants of BCPD and BSA at different temperature were 3.82×106 L·mol-1.The binding site was 1.The thermodynamic parameters indicated that the interaction of BCPD and BSA was mainly driven by hydrophobic force.

关 键 词：N N-二(2-羧基苯基)-2 6-吡啶二甲酰胺 牛血清白蛋白 荧光光谱 

分 类 号：O657.39[理学—分析化学]