激光二极管激光诱导荧光检测器的研制  

Fabrication of A Laser-Induced Fluorescence Detector Using Laser Diode

作　　者：何崇慧 吴晶[2] 王廷海[1] 杨红强[1] 

机构地区：[1]中国石油天然气股份有限公司兰州化工研究中心,甘肃兰州730060 [2]兰州大学化学化工学院,甘肃兰州730000

出　　处：《分析科学学报》2015年第4期554-559,共6页Journal of Analytical Science

基　　金：国家自然科学基金(No.21175062;20935002)

摘　　要：采用紫色激光二极管作为荧光检测器的激发光源,以普通光电倍增管作为光电转换装置,构建共聚焦光学结构的激光诱导荧光检测器。考察了物镜放大倍数、滤光片、光源电流和增益大小等条件对于仪器分析性能的影响,并用荧光胺和FITC衍生的氨基酸、罗丹明类染料、荧光素等物质对灵敏度进行了表征,得到该检测器对荧光胺衍生的亮氨酸的检出限为0.02nmol·L-1,对于罗丹明B和荧光素钠的检出限分别为9.46nmol·L-1和1.02nmol·L-1。该检测器相对文献报道的其它激发光源的检测器,在保持了低成本优势的同时还具有高灵敏度的优点,而且可用于多类染料标记物的分析检测。A laser-induced fluorescence detector was assembled with a commercially available low-cost violet laser diode as the excitation source and photomultiplier tube（PMT）as the photoelectric conversion device which were arranged in a confocal optical configuration.Factors including the magnification of objectives,optical filters,current of the laser diode and gain of the PMT,were investigated according to the performance of the detector.Fluorescamine（FA）and fluorescein isothiocyanate（FITC）derivatized amino acids,rhodamine dyes and fluorescein were used to evaluate the sensitivity of the detector.The limit of detection（LOD）of 0.02nmol·L^-1 was achieved for fluorescamine derivatived leucine,and LODs for rhodamine B and fluorescein sodium was 9.46 and 1.02nmol·L^-1,respectively.Compared with other fluorescence detector,this detector maintains the advantage of low cost,while the sensitivity is much higher and it can also be used for abroad range of dyes for fluorescent labeling.

关 键 词：激光二极管 荧光检测 经济型 芯片电泳 

分 类 号：O657.39[理学—分析化学]