氢化物发生-原子荧光光谱法测定植物样品中的硒  

Determination of Selenium in Plant Samples by Hydride Generation-Atomic Fluorescence Spectrometry

作　　者：胡婷[1] 李文芳[2] 向昌国[1,3] 聂亚文[2] 

机构地区：[1]吉首大学林产化工工程湖南省重点实验室,湖南张家界427000 [2]吉首大学城乡资源与规划学院,湖南张家界427000 [3]吉首大学生态旅游湖南省重点实验室,湖南张家界427000

出　　处：《分析科学学报》2015年第4期579-582,共4页Journal of Analytical Science

基　　金：湖南省自然科学基金(No.07JJ6067);吉首大学校级课题(13JDY055)

摘　　要：本文应用氢化物发生-原子荧光光谱法对植物样品中硒总量的测定方法进行研究。优化了植物样品预处理,选取H2O2-HNO2（1∶5,V/V）体系微波消解;优化了测定中载流与还原剂的浓度,选定载流HCl浓度为15%,还原剂KBH4浓度为1.5%。该方法测定植物样品中的总硒,线性范围为0-100μg/L（r=0.9995）,检出限为0.0906μg/L,回收率在90.0%-112.2%范围,精密度（RSD）小于0.39%。该方法操作简单、灵敏度高、检出限低,适合对植物样品中硒总量的分析测定。A method for determination of selenium in plants by hydride generation atomic fluorescence spectrometry（HG-AFS）has been developed.The pretreatment process for plant samples were optimized.The medium was selected as hydrogen peroxide and nitric acid mixture（1∶5,V/V）.The conditions for microwave dielectric auxiliary and the load flow and concentration of reducing agent were also optimized.The linear range in plant samples is 0-100μg/L（r=0.9995）,the detection limit is0.0906μg/L,the recoveries are 90%-112.2%,and the relative standard derivations are less than0.39%.The method is simple,sensitive and suitable for determination of total selenium in plant samples

关 键 词：氢化物发生原子荧光 植物 载流 还原剂 硒 

分 类 号：O657.31[理学—分析化学]