颐脑解郁方对脑卒中大鼠模型边缘系统脑源性神经营养因子的影响  

Effect of Yinao Jieyu Recipe on Brain Derived Neurotrophic Factor of the Limbic System in PostStroke Model Rats

作　　者：李冬梅[1] 唐启盛[2] 赵瑞珍[2] 李小黎[2] 王戈[2] 杨歆科[2] 

机构地区：[1]北京中医药大学第三附属医院心血管科,北京100029 [2]北京中医药大学第三附属医院脑病科,北京100029

出　　处：《中国中西医结合杂志》2015年第8期988-992,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30973740);国家医药管理局中医药行业专项(No.20080709);北京中医药大学自主选题项目(No.2014-JYBZZ-JS-065)

摘　　要：目的观察脑卒中大鼠边缘系统脑源性神经营养因子（brain derived reurotrophic factor,BDNF）的演变趋势及颐脑解郁方对其干预作用。方法选择雄性Wistar大鼠,经Open-Field行为学评分后,按随机数字表分为正常组、假手术组、多发脑梗塞（multiple cerebral infarction,MCI）组、卒中后抑郁（post-stroke depression,PSD）组、中药组、西药组,其中正常组6只,假手术组7只,余每组各10只。正常组常规饲养。假手术组经颈外动脉推注0.3 mL生理盐水,并灌胃蒸馏水。其余各组均采用同种系微栓子体外注入法,由颈外动脉推注等剂量同种异体血栓栓子;PSD组术后7日予21日慢性不可预知的温和应激（chronic unpredictable mild stress,CUMS）加孤养复制PSD模型;中药组及西药组在MCI模型制备后,分别予颐脑解郁方、尼莫地平干预4周。于干预第1、2、4周进行动态观察边缘系统（海马、下丘脑、杏仁核）BDNF变化及中药干预效果。结果 BDNF免疫组化染色结果显示,与正常组同期比较,MCI组2、4周海马、下丘脑及杏仁核BDNF表达降低（P〈0.01,P〈0.05）,PSD组1-4周各部位BDNF表达降低（P〈0.01）。与MCI组同期比较,PSD组1-4周各部位BDNF表达降低（P〈0.01）。与PSD组同期比较,中药组1-4周各部位BDNF表达升高（P〈0.01）。结论脑卒中大鼠存在BDNF改变,颐脑解郁方能减慢此过程的进展。Objective To observe the evolutionary tendency of brain derived neurotrophic factor （BDNF） of the limbic system in post-stroke model rats and the intervention effect of Yinao Jieyu Recipe （YJR）. Methods Male Wistar rats were randomly divided into the normal control group （n =6）, the sham-operation group （n =7）, the multiple cerebral infarction （MCI） group （n =10）, the post-stroke depression （PSD） group （n =10）, the Chinese medicine （CM） treatment group （n =10）, and the Western medicine （WM） treatment group （n =10） according to random digit table after open-field testing. Rats in the normal control group were routinely fed. 0.3 mL normal saline was intravenously pushing from the external carotid artery to rats in the sham-operation group, and distilled water administered to them by gastrogavage. Each dose allogenic microthrombi were in vitro pushed to rats in the rest groups from the external carotid artery. The PSD model was duplicated by 21-day chronic unpredictable mild stress （CUMS） and single cage feeding in the PSD group 7 days after surgery. After preparing models rats in the CM group and the WM group were administered with Y JR and Nimodipine respectively for 4 successive weeks. Changes of BDNF and the intervention effect of Y JR were observed at week 1,2, and 4 after intervention. Results Immunohistochemical results of BDNF showed, compared with the normal control group, expression levels of BDNF in the hippocampus, hypothalamus, and amygdala decreased in the MCI group at week 2 and 4 （P 〈0.01, P 〈0.05） ; expression levels of BDNF in each part decreased in the PSD group at week 1 -4 （P 〈0.01 ）. Compared with the MCI group, expression levels of BDNF in each part decreased in the PSD group at week 1 -4 （P 〈0.01 ）. Compared with the PSD group, expression levels of BDNF in each part increased in the CM group at week 1 -4 （P 〈0.01 ）. Conclusion BDNF changes existed in post-stroke model rats, and Y JR could slow down this progress.

关 键 词：脑卒中 卒中后抑郁 边缘系统 脑源性神经营养因子 颐脑解郁方 

分 类 号：R285.5[医药卫生—中药学]