芫花根醇提颗粒联合甲基泼尼松龙对脊髓损伤大鼠BDNF、NMDA表达及行为学的影响  

Effect of Herba Lycopodii Alcohol Extracted Granule Combined Methylprednisolone on Expression Levels of BDNF and NMDA and Behavior of Traumatic Spinal Cord Injury Rats

作　　者：许争光[1] 杨峻[1] 吕志平[1] 王廷华[2] 李晓松[1] 刘江华[1] 赵楠[3] 习阳彦斌[2] 

机构地区：[1]云南省玉溪市中医医院,云南653100 [2]昆明医学院神经科学研究所,昆明650031 [3]云南省昆明市第一人民医院神经外科,昆明650031

出　　处：《中国中西医结合杂志》2015年第8期1004-1010,共7页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：玉溪市科技合作专项计划项目

摘　　要：目的研究芫花根醇提颗粒（金腰带）联合甲基泼尼松龙对脊髓损伤（spinal cordinjury,SCI）大鼠行为学、脑源性神经营养因子（brain derived neurotrophic factor,BDNF）表达及N-甲基-D-门冬氨酸（N-methyl-D-aspartate,NMDA）水平的影响。方法 SD成年雄性大鼠,随机分为假手术组、脊髓挫伤组（模型组）、金腰带组[灌胃金腰带50 mg/（kg·d）,共5天]、甲基泼尼松龙组（脊髓钝挫伤术后8 h内肌肉注射50 mg/kg甲基泼尼松龙,此后甲基泼尼松龙剂量每天减少10 mg/kg,共5天）及联合组（金腰带和甲基泼尼松龙联合干预）。各组大鼠均在术后（0、3、7、28天）进行后肢行为学评价[神经行为学（Basso Beattie and Bresnahan,BBB）评分]。术后13天,每组取8只大鼠,麻醉后取损伤脊髓T（8-10）液氮中保存,采用[-3H]MK-801放射性配基分析法测定NMDA受体亲和力（Kd）和最大结合数量（Bmax）;取术后28天大鼠损伤脊髓,采用免疫组织化学法检测BDNF表达。结果与假手术组比较,模型组腹角和背角BDNF阳性神经元数量升高,Bmax（470±34）升高,Kd降低,术后3-28天BBB评分降低,差异有统计学意义（P〈0.05）。与模型组比较,各给药组BDNF阳性神经元数量及Kd升高,术后3-28天BBB评分升高（P〈0.05）,Bmax[甲基泼尼松龙组：660±15,金腰带组：646±25,联合组：510±21]降低（P〈0.05）。与甲基泼尼松龙组比较,金腰带组及联合组BDNF阳性神经元数量及Kd升高（P〈0.05）,术后7-28天BBB评分升高（P〈0.05）,Bmax降低（P〈0.05）。与金腰带组比较,联合组BDNF阳性神经元数量及Kd升高（P〈0.05）,Bmax降低（P〈0.05）。结论金腰带能有效改善SCI大鼠后肢运动功能,增加脊髓组织中BDNF的表达,通过影响脊髓NMDA受体减轻脊髓继发性损伤,对SCI有一定的治疗与保护作用,效果优于甲基泼尼松龙且与其有协同作用。Objective To study different effects of Herba Lycopodii （HL） Alcohol Extracted Granule combined methylprednisolone on behavioral changes, brain derived neurotrophic factor （BDNF） expression levels, and N-methyl-D-aspartate （NMDA） receptor levels in rats with spinal cord injury （SCI）. Methods Male adult SD rats were randomly divided into five groups, i.e., the sham-operation group, the model group, the HL treatment group, the methylprednisolone treatment group, the HL ± methylprednisolone treatment group. Rats in the HL treatment group were intragastrically administered with HL at the daily dose of 50 mg/kg for 5 successive days. Rats in the methylprednisolone treatment group were intramuscularly injected with 50 mg/kg methylprednisolone within 8 h after spinal cord contusion, and then the dose of methylprednisolone was reduced for 10 mg/kg for 5 successive days. Rats in the HL ± methylprednisolone treatment group received the two methods used for the aforesaid two groups. Basso Beattie and Bresnahan （BBB） score （for hindlimb motor functions） were assessed at day 0, 3, 7, and 28 after operation. At day 13 after SCI, injured spinal T8-10 was taken from 8 rats of each group and stored in liquid nitrogen. The N- methyI-D-aspartate （NMDA） receptor affinity （Kd） and the maximal binding capacity （Bmax） were determined using [^3H]MK-801 radioactive ligand assay. Rats＇ injured spinal cords were taken for immunohistochemical assay at day 28 after SCI. Expression levels of BDNF in the ventral and dorsal horn of the spinal cord were observed. Results Compared with the sham-operation group, the number of BDNF positive neurons in the ventral and dorsal horn of the spinal cord increased in the model group, Bmax increased （470 ±34）, Kd decreased, and BBB scores decreased at day 3 -28 （all P 〈0.05）. Compared with the SCI model group, the number of BDNF positive neurons and Kd increased, BBB scores at day 3 -28 increased （P 〈0.05） in each medicated group. Bmax was （660 ±1

关 键 词：芫花根醇提颗粒 甲基泼尼松龙 脊髓钝挫伤 神经行为学评分 脑源性神经营养因子 N-甲基-D-门冬氨酸 

分 类 号：R285.5[医药卫生—中药学]