华蟾素抑制人胃癌BGC-823细胞体外侵袭、迁移的机理  

Inhibitory effect of Cinobufacin on the invasion and migration of human gastric carcinoma BCG-823 cells and its mechanism in vitro

作　　者：乔翠霞[1] 张新峰[2] 蔡小平[1] 程旭锋[3] 

机构地区：[1]河南省中医药研究院附属医院肿瘤科,河南郑州450004 [2]河南省肿瘤医院中西医科,河南郑州450000 [3]河南中医学院第一附属医院乳腺科,河南郑州450000

出　　处：《中成药》2015年第8期1655-1659,共5页Chinese Traditional Patent Medicine

基　　金：河南省中医药管理局课题(2013ZY02026)

摘　　要：目的探讨华蟾素(Cinobufacin)体外抑制人胃癌BGC-823细胞侵袭的作用及其机制。方法以0.156 mg/L和0.313 mg/L华蟾素体外作用人胃癌BGC-823细胞,采用MTT法检测华蟾素对BCG-823细胞生长的抑制效果,Transwell小室法检测细胞的迁移和侵袭能力,Western blot法检测BGC-823细胞中NF-κB p56、MMP-9蛋白的表达量。结果华蟾素可浓度依赖性抑制体外BGC-823细胞增殖、迁移和侵袭,同时下调BGC-823细胞中NF-κB和MMP-9蛋白表达。结论华蟾素体外可抑制胃癌的转移,该作用可能通过下调NF-κB、MMP-9的表达相关。AIM To investigate the anti-invasive effect of Cinobufacin on the human gastric carcinoma BCG-823 cells and its mechanism in vitro. METHODS BCG-823 cells were treated by 0. 156 mg / L and0. 313 mg / L Cinobufacin. Their inhibitory action was measured by MTT assay. The migration and invasive ability of BCG-823 cells were measured by Transwell assay. Western blot analysis was performed on NF-κB p56,MMP-9 in BCG-823 cells. RESULTS Cinobufacin significantly inhibited the growth,migration and invasive ability of BCG-823 cells in a dose-dependent manner. The expressions of NF-κB and MMP-9 were suppressed and the MMP-9 / TIMP-1 ratio reduced after the treatment with Cinobufacin. CONCLUSIONS Cinobufacin can inhibit the migration and invasion of BCG-823 cells probably by down-regulating the expression of NF-κB and MMP-9.

关 键 词：人胃癌BGC-823细胞 华蟾素 NF-ΚB MMP-9 侵袭 迁移 

分 类 号：R966[医药卫生—药理学]