正交设计联用Box-Behnken效应面法优选复方石斛清肺散提取工艺  

Extraction optimization for Compound Shihu Qingfei Powder by orthogonal design and Box-Behnken design-response surface

作　　者：谢伟杰[1] 张永萍[1] 徐剑[1] 吴静澜[1] 陈鑫瑀 魏中旭 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《中成药》2015年第8期1683-1688,共6页Chinese Traditional Patent Medicine

基　　金：贵州省科技厅项目(黔科合重G字[2012]4001);贵阳中医学院研究生教育创新项目(ZYY14024)

摘　　要：目的采用正交设计和Box-Behnken响应曲面法优选复方石斛清肺散最佳提取工艺。方法出膏率、橙皮苷含有量为评价指标,通过正交设计初步优选提取工艺,Box-Behnken效应面法进一步优化工艺,从可行性和经济性确定最终提取工艺条件。结果正交设计优选的最佳工艺为12倍量50%乙醇,回流提取3次,每次1.5 h;效应面法优化工艺为14倍量32%乙醇,回流提取3次,每次1.5 h。结论对比验证试验表明,Box-Behnken效应面法优化后提取溶剂用量稍增加,但乙醇体积分数显著降低,经济成本将大幅降低。AIM To establish and optimize the extraction of Compound Shihu Qingfei Powder（ CSQP） by the orthogonal design and Box-Behnken design-response surface. METHODS The extraction of CSQP was initiated through the orthogonal design,and was then furtherly optimized by Box-Behnken design with the hesperidin content and the paste-forming rate assigned as indices. RESULTS The optimum extraction of orthogonal design was triplicated 12-fold use of 50% alcohol,1. 5 h for each time. The optimum extraction of Box-Behnken design was triplicated 14-fold use of 32% alcohol,1. 5 h for each time. CONCLUSION The extraction method by Box-Behnken design takes its economical advantage,with reference to the extraction by orthogonal design,in terms of a lower alcohol concentration requirement,in spite of an increased amount of alcohol consumption.

关 键 词：复方石斛清肺散 Box-Behnken响应曲面法 正交设计 橙皮苷 出膏率 提取 

分 类 号：R284.2[医药卫生—中药学]