HPLC-DAD法同时测定理冲生髓饮中7种亲脂性成分  

Simultaneous determination of seven lipophilic constituents in Lichong Shengsui Drink by HPLC-DAD

作　　者：王艳宏[1] 尚冬雪[1] 李洋[1] 赵永伟[1] 张艳[2] 韩凤娟[2] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《中成药》2015年第8期1713-1717,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(81273788)

摘　　要：目的建立RP-HPLC法同时测定理冲生髓饮中亲脂性成分的分析方法。方法理冲生髓饮超临界萃取,HPLC法分析采用Dikma Diamonsil-C18色谱柱（250 mm×4.6 mm,5μm）,以0.1%磷酸水溶液-乙腈为流动相梯度洗脱,体积流量为1 m L/min,检测波长210 nm。结果姜黄素、莪术二酮、莪术醇、牻牛儿酮、呋喃二烯、亚油酸、β-榄香烯分别在0.16-8.14μg/m L、0.27-13.44μg/m L、0.10-4.98μg/m L、0.18-9.10μg/m L、0.08-4.06μg/m L、0.21-10.59μg/m L、0.28-14.18μg/m L范围内线性关系良好。平均加样回收率在96.76%-100.38%之间。结论本方法能够准确测定理冲生髓饮7种亲脂性成分。AIM To establish an HPLC-DAD method for simultaneously determining the contents of curcumin,curcumol,cermacrone, curdione, furanodiene, β-elemene and linoleic acid in Lichong Shengsui Drink（ LCSSD）. METHODS HPLC analysis of LCSSD methanolic extract was performed on Dikma Diamonsil-C18（ 250 mm × 4. 6 mm,5 μm） column with the mobile phase of 0. 1% phosphoric acid aqueous solution as phase A and acetonitrile as phase B in gradient elution manner. The volumetric flow rate was 1 m L / min and the detection wavelength was set at 210 nm. RESULTS Every constituent was separated perfectly and good liner relationships between peak area and mass concentration were obtained in the ranges of 0. 16- 8. 14 μg / m L,0. 27- 13. 44μg /m L,0. 10- 4. 98 μg /m L,0. 18- 9. 10 μg /m L,0. 08- 4. 06 μg /m L,0. 21- 10. 59 μg /m L and 0. 28-14. 18 μg / m L,respectively. The average recovery rate was within the ranges of 96. 76%- 100. 38%. CONCLUSION This method is accurate and reliable for the determination of above-mentioned seven lipophilic constituents in LCSSD.

关 键 词：理冲生髓饮 姜黄素 莪术醇 牻牛儿酮 莪术二酮 呋喃二烯 β-榄香烯 亚油酸 

分 类 号：R927.2[医药卫生—药学]