HPLC法快速筛查中成药中三七/人参茎叶代替根和根茎  

Rapid screening of stems and leaves in the place of roots and rhizomes of Panax ginseng and Panax notoginseng by HPLC

作　　者：费毅琴 杨萍 肖凌 侯俊杰 聂晶 

机构地区：[1]湖北省食品药品监督检验研究院,湖北武汉430064

出　　处：《中成药》2015年第8期1730-1733,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立HPLC法快速筛查中成药中以三七/人参茎叶代替根和根茎投料。方法采用HPLC法对含三七/人参的复方丹参片、三七片/胶囊、参苓白术散进行筛查,检测其中的人参皂苷Rb3,Rg1和Re,色谱柱为Agilent Eclipse Plus C18(5μm,4.6 mm×250 mm),流动相为乙腈-水梯度洗脱,体积流量为1.0 m L/min,检测波长为203 nm。结果若检出人参皂苷Rb3,则为三七茎叶投料;若人参皂苷Rg1/Re比值小于5,则为人参茎叶投料。复方丹参片存在以三七/人参茎叶非法投料的现象。结论本法较原补充检验中TLC法准确度高,不存在假阳性的干扰。AIM To establish an HPLC method to rapidly identify the stems and leaves in the place of root and rhizome of Panax ginseng and Panax notoginseng,which should not be added in Chinese patent medicines.METHODS HPLC was used to analyze ginsenoside Rb3,Rg1 and Re in Compound Danshen Tablets,Sanqi Tablets / Capsules and Shenlin Baizhu Powder. The column was Agilent Eclipse Plus C18（ 5 μm,4. 6 mm × 250 mm）with acetonitrile-water as the mobile phase in a gradient manner. The detection wavelength was set at 203 nm with1. 0 m L / min of flow rate. RESULTS If ginsenoside Rb3 was checked out it showed that stems and leaves of P.notoginseng was added in. If the specific value of ginsenoside Rg1 and Re was less than five it showed that stems and leaves of Panax ginseng was added in. This examination revealed that there existed adulteration in Compound Danshen Tablets. CONCLUSION The method is more accurate than TLC in supplemental testing method,and excludes the possibility of the false positive.

关 键 词：三七 人参 茎叶 根和根茎 HPLC 快速筛查 中成药 

分 类 号：R927.2[医药卫生—药学]